Antimicrobial resistance is a public health concern. Understanding any role that urban seagulls may have as a reservoir of resistant bacteria could be important for reducing transmission. This study investigated fecal Escherichia coli isolates from seagulls (herring gulls and lesser black-backed gulls) to determine the prevalence of extended-spectrum cephalosporin-resistant (ESC-R) and fluoroquinolone-resistant E. coli among gull species from two cities (Taunton and Birmingham) in the United Kingdom (UK). We characterized the genetic background and carriage of plasmid-mediated resistance genes in extended-spectrum β-lactamase (ESBL)-producing E. coli obtained from these birds. Sixty ESC-R E. coli isolates were obtained from 39 seagulls (39/78, 50%), of which 28 (28/60, 46.7%) were positive for plasmid-mediated CTX-M and/or AmpC β-lactamase resistance genes. Among these, bla_CTX-M-15, bla_CTX-M-14, and bla_CMY-2 predominated. Three isolates belonging to the B2-ST131 clone were detected, of which two harbored bla_CTX-M-15 (typed to C2/H30Rx) and one harbored bla_CTX-M-27 and was typed to C1/H30-R (recently described as the C1-M27 sublineage). The plasmid-mediated quinolone resistance (PMQR) gene carriage prevalence (11.7%) consisted of aac(6′)-Ib-cr and qnrB genes. No carbapenem or colistin resistance genes were detected. Urban seagulls in the UK are colonized and can spread major antimicrobial-resistant E. coli isolates harboring ESBL and PMQR determinants, including clinically important strains such as the pandemic clone B2-ST131 and the C1-M27 subclade. This is the first report of ST131-C1-M27 subclade in wildlife in the UK and in seagulls worldwide.

中文翻译：

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来自英国的城市海鸥中，产ESBL的大肠杆菌ST131的出现，包括H30-Rx和C1-M27亚克隆。

抗菌素耐药性是公共卫生问题。了解城市海鸥作为抗性细菌库的任何作用对于减少传播很重要。这项研究调查了从两个海鸥物种（汤顿（Taunton ））中从海鸥（鲱鸥和较小的黑背鸥）分离到的粪便大肠杆菌，以确定广谱头孢菌素耐药性（ESC-R）和氟喹诺酮耐药性大肠杆菌的流行情况和伯明翰）。我们表征了从这些鸟中获得的广谱β-内酰胺酶（ESBL）生产大肠杆菌中质粒介导的抗性基因的遗传背景和运输。六十种ESC-R大肠杆菌从39只海鸥（39/78，50％）获得了分离株，其中28（28/60，46.7％）对质粒介导的CTX-M和/或AmpCβ-内酰胺酶耐药基因呈阳性。其中，bla _CTX-M-15，bla _CTX-M-14和bla _CMY-2占主导。检测到三个属于B2-ST131克隆的分离株，其中两个带有bla _CTX-M-15（类型为C2 / H30Rx），一个带有bla _CTX-M-27，类型为C1 / H30-R（最近有描述）作为C1-M27子系）。质粒介导的喹诺酮耐药性（PMQR）基因携带率（11.7％）由aac（6'）-Ib-cr和qnrB组成基因。未检测到碳青霉烯或大肠粘菌素抗性基因。英国的城市海鸥已定居，并可以传播带有ESBL和PMQR决定簇的主要抗微生物大肠杆菌分离株，包括临床上重要的菌株，例如大流行性克隆B2-ST131和C1-M27亚克隆。这是ST131-C1-M27子小节在英国野生生物和全球海鸥中的首次报道。