Foodborne illnesses caused by the consumption of food contaminated with foodborne pathogens at infectious doses are becoming a common health issue throughout the world. Metal nanoparticles with potential antimicrobial properties are an area that can be explored to discover novel antimicrobial agents. The traditional synthesis methods of metal nanoparticles involve the use of toxic chemicals and the generation of harmful byproducts. In this study, a greener method to synthesize silver nanoparticles (AgNPs) with potential antimicrobial properties was investigated. The aqueous extract of the medicinal plant Phyla dulcis Trev. (verbenaceae) was used as the reducing and stabilizing agent to synthesize AgNPs using microwave irradiation. The formation of AgNPs was confirmed using ultraviolet-visible spectroscopy by the appearance of characteristic surface plasmon resonance peaks in the 430–440 nm wavelength range. The size and stability of the AgNPs were studied using Zetasizer nano-series for 5 weeks after synthesis. The average particle size remained between 63 and 76 nm during the first 4 weeks and increased to 114 nm in the fifth week showing possible aggregation after the fourth week. The zeta potential remained between −20 and −24 mV throughout the 5 weeks showing relatively good stability. Scanning electron microscopy/energy dispersive X-ray spectroscopy showed the association of phytoconstituents with the AgNPs. X-ray photoelectron spectroscopy analysis confirmed the formation of metallic nanoparticles starting from silver nitrate. Finally, the AgNPs were tested to be effective against Escherichia coli O157:H7 (ATCC 43888), Salmonella Typhimurium (novobiocin and nalidixic acid resistant strain), Listeria monocytogenes (4b; ATCC 19115), and Staphylococcus aureus (ATCC 6538) strains, which are known to be common foodborne pathogens.

中文翻译：

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使用 Phyla dulcis 植物提取物绿色合成具有抗菌特性的银纳米颗粒。

因食用受食源性病原体污染的食物而导致的食源性疾病正在成为全世界常见的健康问题。具有潜在抗菌特性的金属纳米颗粒是一个可以探索发现新型抗菌剂的领域。金属纳米粒子的传统合成方法涉及使用有毒化学品和产生有害副产物。在这项研究中，研究了一种更环保的方法来合成具有潜在抗菌性能的银纳米粒子 (AgNPs)。药用植物Phyla dulcis的水提物特雷夫。（马鞭草科）作为还原剂和稳定剂，使用微波辐射合成 AgNPs。通过在 430-440 nm 波长范围内出现特征表面等离子体共振峰，使用紫外-可见光谱证实了 AgNPs 的形成。合成后使用 Zetasizer 纳米系列研究 AgNP 的尺寸和稳定性 5 周。平均粒径在前 4 周保持在 63 和 76 nm 之间，并在第五周增加到 114 nm，表明第四周后可能发生聚集。zeta 电位在整个 5 周内保持在 -20 和 -24 mV 之间，显示出相对良好的稳定性。扫描电子显微镜/能量色散 X 射线光谱显示植物成分与 AgNPs 的关联。X 射线光电子能谱分析证实了从硝酸银开始形成金属纳米颗粒。最后，测试了 AgNPs 对大肠杆菌O157:H7 (ATCC 43888)、鼠伤寒沙门氏菌（新生霉素和萘啶酸抗性菌株）、单核细胞增生李斯特菌（4b；ATCC 19115）和金黄色葡萄球菌(ATCC 6538) 菌株，这些都是已知的常见食源性病原体。