In order to analyze the mycotoxins in corn, the modified QuEChERS high-performance liquid chromatography was applied to extract, clean up, and detect mycotoxins in a nonpolar system. The impurities such as fat and protein were removed from the corn sample, and the impurities in the sample solution have almost no effect on extraction efficiency. The proposed method leads to a greater choice of mycotoxin-extraction solvents in high-fat-solid samples. An appropriate extraction solvent was selected, the pretreatment conditions were optimized, and the extraction and cleanup of mycotoxins in high-fat solids were enhanced. By changing the experiment parameters, this method can be further used for the extraction and analysis of mycotoxins in complex samples (nonfat, low-fat, or high-fat). This method achieves good linearity in the range of 2.5–1000 μg/kg with the correlation coefficients for all analytes in the range of 0.9975 to 0.9989. The acceptable standard deviations for intraday and interday precision were 1.8–4.3% and 3.2–5.2%, respectively, with recoveries from 89.7 to 105.9%.

中文翻译：

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基于混合吸附剂净化玉米中霉菌毒素的改良 QuEChERS

为了分析玉米中的霉菌毒素，采用改良的 QuEChERS 高效液相色谱法在非极性系统中提取、净化和检测霉菌毒素。玉米样品中脂肪、蛋白质等杂质被去除，样品溶液中的杂质对提取效率几乎没有影响。所提出的方法为高脂肪固体样品中的霉菌毒素提取溶剂提供了更多选择。选择合适的提取溶剂，优化前处理条件，加强对高脂固体中霉菌毒素的提取和净化。通过改变实验参数，该方法可进一步用于复杂样品（脱脂、低脂或高脂）中霉菌毒素的提取和分析。该方法在 2 范围内实现了良好的线性。5–1000 μg/kg，所有分析物的相关系数在 0.9975 到 0.9989 范围内。日内和日间精密度的可接受标准偏差分别为 1.8-4.3% 和 3.2-5.2%，回收率为 89.7% 至 105.9%。