This study investigated the potential of the bacterium Stenotrophomonas maltophilia UCP 1601 to produce a new biomolecule with emulsifying properties by determining the hemolytic activity, obtaining a halo of 9 mm in blood agar. Fermentations were carried out in saline mineral medium supplemented with 10% waste soybean oil (WSO) and different concentrations of glucose, peptone, ZnCl₂, and MgSO₄, according to a 2⁴ full-factorial design. The results showed that the best results were obtained in condition 6 (medium composed of 4% glucose, 1% peptone, 2.72% ZnCl₂, and 2.46% MgSO₄), with excellent high emulsification index of 82.74%, using burned motor oil. The emulsifying property of the biomolecule produced was confirmed by the emulsification index of 78.57, 54.07, and 58.62%, using soybean, corn, and diesel oils, respectively, and the stability at different values of pH, temperature, and NaCl concentrations. The yield of the produced bioemulsifier was 2.8 g/L, presenting an anionic character and polymeric nature (37.6% lipids, 28.2% proteins, and 14.7% carbohydrates), confirmed by FTIR. The new bioemulsifier demonstrated promising potential for bioremediation of hydrophobic contaminants in the environment, since it had the ability to reduce the viscosity of WSO and burned motor oil, as well as excellent dispersion capacity of the burned motor oil in water (69.94 cm² of oil displacement area), and removing 71.7% of this petroleum derivative from sandy soil.

中文翻译：

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嗜麦芽窄食单胞菌UCP 1601在生物乳化剂生产中对农业工业废物的生物转化及其在生物修复过程中的应用

这项研究通过确定溶血活性来研究嗜麦芽窄食单胞菌UCP 1601产生具有乳化特性的新生物分子的潜力，并在血琼脂中获得9毫米的光晕。根据2 ⁴全因子设计，在补充有10％废大豆油（WSO）和不同浓度的葡萄糖，蛋白ept，ZnCl ₂和MgSO _4的盐矿物培养基中进行发酵。结果表明，在条件6（由4％葡萄糖，1％蛋白ept，2.72％ZnCl ₂和2.46％MgSO ₄组成的介质）中获得了最佳结果。），使用燃烧的机油具有极高的82.74％的高乳化指数。分别使用大豆油，玉米油和柴油的乳化指数分别为78.57、54.07和58.62％，以及在不同pH，温度和NaCl浓度下的稳定性，证实了所生产生物分子的乳化特性。FTIR证实，生产的生物乳化剂的产量为2.8 g / L，具有阴离子特性和聚合物性质（37.6％的脂质，28.2％的蛋白质和14.7％的碳水化合物）。新型生物乳化剂具有降低WSO和燃烧的机油粘度的能力，并且具有出色的燃烧后的机油在水中（69.94 cm ^2）的分散能力，因此具有在环境中疏水性污染物进行生物修复的潜力。 石油驱替面积的百分比），并从沙土中去除71.7％的石油衍生物。