Acrylamide (AA) is a carcinogenic substance found in food, cigarette smoke and in an environment exposed to acrylamide. This study aims to analyze AA levels in dried blood spot (DBS) samples of lung cancer patients with smoking record, without smoking record, and also in the negative blank. Analysis of AA levels was determined by liquid chromatography tandem mass spectrometry (LC-MS/MS) and DBS extraction using protein precipitation techniques. Mass detection was done using positive electron spray ionization (ESI) and multiple reaction monitoring (MRM) type with m/z values of 71.99 > 55.23 for acrylamide and m/z 260.16 > 116.04 for propranolol as the internal standard. AA levels in lung cancer patients with smoking record is in the range of 4.670 μg/mL to 11.986 μg/mL. AA levels in lung cancer patients without smoking record is in the range of 2.041 μg/mL to 12.702 μg/mL. Data on AA levels on negative blanks is in the range of 2.72 μg/mL to 3.51 μg/mL. The results of the independent sample t-test () showed that AA levels in patients with smoking record and those without smoking record did not differ significantly. Then, the Mann-Whitney test was performed between the lung cancer group and the negative blank group and a significant difference was found between the two groups ().

中文翻译：

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超高效液相色谱串联质谱法分析肺癌患者干血斑中的丙烯酰胺。

丙烯酰胺（AA）是一种致癌物质，存在于食物，香烟烟雾中以及暴露于丙烯酰胺的环境中。本研究旨在分析有吸烟记录，无吸烟记录以及阴性空白的肺癌患者的干血斑（DBS）样品中的AA水平。通过液相色谱串联质谱法（LC-MS / MS）和使用蛋白质沉淀技术的DBS提取来测定AA水平。使用正电子喷雾电离（ESI）和多反应监测（MRM）类型进行质量检测，丙烯酰胺和m / z的m / z值为71.99> 55.23普萘洛尔作为内标物为260.16> 116.04。在肺癌患者AA水平与吸烟记录是在4.670的范围 μ克/ mL至11.986  μ克/毫升。在肺癌患者AA水平，而不吸烟记录是的2.041的范围内 μ克/ mL至12.702  μ克/毫升。数据上负坯料AA水平是在2.72的范围内 μ克/ mL至3.51  μ克/毫升。独立样本t检验的结果（）表明有吸烟记录的患者和没有吸烟记录的患者的AA水平没有显着差异。然后，在肺癌组和阴性空白组之间进行了Mann-Whitney测试，发现两组之间存在显着差异（）。