A novel preconcentration/separation method for simultaneous sorption-spectrophotometric determination of anionic food dyes Sunset Yellow and Tartrazine is proposed. The method is based on preconcentration of the dyes using solid phase extraction on a cartridge filled with silica chemically modified with C16 groups from aqueous solution at pH 1 followed by elution with water/acetonitrile mixture containing 2 mmol·L⁻¹ KH₂PO₄ adjusted to pH 3 with a step gradient of acetonitrile content. This elution allows quantitative separation of the dyes which makes their individual spectrophotometric determination possible. The detection limits for Tartrazine and Sunset Yellow are 0.15 and 0.11 μg·mL⁻¹ and the linearity range is 2–20 μg·mL⁻¹. The method is applied for analysis of beverages. The recovery of dyes is higher than 97% at the relative standard deviation not exceeding 10%.

中文翻译：

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使用单个试剂盒同时富集和分离，同时进行光度检测，可同时测定合成食品染料

提出了一种同时富集-分光光度法同时测定阴离子食用染料日落黄和酒石黄的新方法。该方法是基于使用填充有二氧化硅在pH 1从水溶液C16基团化学改性的药筒上的固相萃取的染料的预浓缩，随后用水/乙腈含有2mmol·L混合物^-1 KH ₂ PO ₄调节乙腈含量的逐步梯度调节至pH 3。该洗脱允许染料的定量分离，这使得它们的分光光度法测定成为可能。为柠檬黄和日落黄的检测限是0.15和0.11  μ克·ML ^-1和线性范围是2-20  μ克·ML ^-1。该方法适用于饮料分析。在相对标准偏差不超过10％的情况下，染料的回收率高于97％。