In two decades of development, impressive strides have been made for automating basic laboratory operations in droplet-based microfluidics, allowing the emergence of a new form of high-throughput screening and experimentation in nanoliter to femtoliter volumes. Despite advancements in droplet storage, manipulation, and analysis, the field has not yet been widely adapted for many high-throughput screening (HTS) applications. Broad adoption and commercial development of these techniques require robust implementation of strategies for the stable storage, chemical containment, generation of libraries, sample tracking, and chemical analysis of these small samples. We discuss these challenges for implementing droplet HTS and highlight key strategies that have begun to address these concerns. Recent advances in the field leave us optimistic about the future prospects of this rapidly developing technology.

中文翻译：

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液滴微流控技术的高通量筛选：透视主要挑战和未来前景。

在过去的二十年的发展中，基于液滴的微流控技术在自动化基础实验室操作方面取得了令人瞩目的进步，从而出现了从纳升到飞升体积的新型高通量筛选和实验形式。尽管液滴存储，处理和分析方面取得了进步，但该领域尚未广泛适用于许多高通量筛选（HTS）应用。这些技术的广泛采用和商业开发要求稳健地实施策略，以稳定地存储，化学容纳，生成库，跟踪样品以及对这些小样品进行化学分析。我们讨论了实现液滴HTS的这些挑战，并重点介绍了已开始解决这些问题的关键策略。