Eulachon Thaleichthys pacificus, a culturally and ecologically important anadromous smelt (Family Osmeridae), ranges from Northern California to the southeast Bering Sea. In recent decades, some populations have experienced declines. Here we use a contig-level genome assembly combined with previously published RADseq-derived markers to construct an amplicon panel for eulachon. Using this panel, we develop a filtered genetic baseline of 521 variant loci genotyped in 1,989 individuals from 14 populations ranging from Northern California through Central Alaska. Consistent with prior genetic studies, the strongest separation occurs among three main regions: from Northern California up to and including the Fraser River; north of the Fraser River to southeast Alaska; and within the Gulf of Alaska. Separating the Fraser River from southern US populations, and refining additional substructure within the central coast may be possible in mixed-stock analysis; this will be addressed in future work. The amplicon panel outperformed the previous microsatellite panel, and thus will be used in future mixed-stock analyses of eulachon in order to provide new insights for management and conservation of eulachon.

中文翻译：

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使用直接扩增子测序的单核苷酸多态性，从北加利福尼亚到阿拉斯加的Eulachon Thaleichthys pacificus的种群结构

Eulachon Thaleichthys pacificus，是一种具有文化和生态学意义的重要冶炼厂（s鱼科），范围从北加利福尼亚到白令海东南。在最近的几十年中，一些人口下降了。在这里，我们使用重叠群水平的基因组组装与以前发布的RADseq衍生的标记相结合，构建了Eulachon的扩增子面板。使用该小组，我们开发了521个变异基因座的基因型筛选基线，该基因型在从北加利福尼亚到阿拉斯加中部的14个人群的1,989个个体中进行了基因分型。与先前的遗传研究一致，最强的分离发生在三个主要区域之间：从北加利福尼亚到弗雷泽河，包括弗雷泽河；弗雷泽河以北，阿拉斯加东南部；在阿拉斯加湾内。将弗雷泽河与美国南部居民区分开来，在混合储层分析中，有可能在中部海岸细化其他结构；这将在以后的工作中解决。扩增子面板的性能优于以前的微卫星面板，因此将在未来的欧拉康混合库存分析中使用，从而为欧拉康的管理和保护提供新见解。