Abstract The aim of this study is to investigate the causes of the different response of Amide I and Amide II bands in neuronal-like cells under an applied magnetic field at extremely low frequency and intensity. A Lumos IR microscope of Bruker Optics coupled with a FTIR spectrometer equipped with a wide-band liquid nitrogen cooled mercury–cadmium–telluride detector were used to acquire images and FTIR spectra of SH-SY5Y neuronal-like cells under exposure to 50 Hz magnetic fields at the intensity of 1.4 mT, generated by two Helmholtz coils driven by an AC voltage and placed into an incubator in a 5% CO2/95% air humidified at 37 °C. Using this experimental setup resulted that the Amide II vibration band in neuronal-like cells increased significantly more than Amide I band after 6 h exposure to 50 Hz magnetic fields. This result can be explained assuming that the following mechanisms occurred simultaneously after exposure to 50 Hz magnetic fields: the alignment of proteins α-helices in cellular membrane toward the direction of applied magnetic field and a significant increasing of N-H bending vibration in proteins structure.

中文翻译：

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SH-SY5Y神经元样细胞在50Hz磁场下N-H弯曲振动与α-螺旋极化之间的竞争

摘要 本研究旨在探讨在极低频率和强度的外加磁场下神经元样细胞中酰胺I和酰胺II带不同响应的原因。Bruker Optics 的 Lumos IR 显微镜与配备宽带液氮冷却汞-镉-碲探测器的 FTIR 光谱仪相结合，用于获取暴露于 50 Hz 磁场下的 SH-SY5Y 神经元样细胞的图像和 FTIR 光谱强度为 1.4 mT，由交流电压驱动的两个亥姆霍兹线圈产生，并放置在 5% CO2/95% 空气中的培养箱中，在 37 °C 下加湿。使用该实验装置导致神经元样细胞中的 Amide II 振动带在暴露于 50 Hz 磁场 6 小时后显着高于 Amide I 带。