Abstract

We determined the complete mitochondrial genome of Batillaria zonalis. The B. zonalis mitochondrial genome is 15748 bp in length, comprising 13 protein-coding genes, 22 transfer RNA genes, and two ribosomal RNA genes. The nucleotide composition for B. zonalis is 17.71% of C, 16.74% of G, 34.99% of T, and 30.3% of A. In 13 protein-coding genes, all genes start with ATG. For the stop codon, the cox2 gene stops with TTC, the cytb, nad1, nad2 genes stop with TAG, the other nine genes are with TAA. Of these 37 genes identified, nine protein-coding genes and six transfer RNA genes are encoded on the heavy strand and the other genes on the light strand. The phylogenetic tree was constructed based on 13 protein-coding genes of the B. zonalis and other 19 Gastropoda species, Sepia latimanus as outgroup using the Neighbour-joining method. The result showed that B. zonalis is most closely related to the Tylomelania sarasinorum in Cerithioidea. We believe that this result will be helpful for the study of population genetic and phylogenetic analysis of the family Batillariidae.

摘要

我们确定了Batillaria zonalis的完整线粒体基因组。所述B. zonalis线粒体基因组的长度为15748个碱基，其包括13个蛋白质编码基因，22转移RNA的基因，和两个核糖体RNA基因。小带芽孢杆菌的核苷酸组成为C的17.71％，G的16.74％，T的34.99％和A的30.3％。在13个蛋白质编码基因中，所有基因均以ATG开头。对于终止密码子，cox2基因以TTC终止，cytb，nad1，nad2基因以TAG终止，其他九个基因以TAA终止。在确定的这37个基因中，有9个蛋白质编码基因和6个转移RNA基因编码在重链上，其他基因编码在轻链上。系统发育树是根据纬带双歧杆菌的13个蛋白质编码基因构建的和其他19个腹足纲动物种类，棕褐色的乌贼墨作为邻群方法。结果表明，B. zonalis最密切相关的Tylomelania sarasinorum在蟹守螺总科。我们认为，该结果将有助于研究巴蒂科的种群遗传和系统发育分析。