International Journal of Environmental Health Research ( IF 3.2 ) Pub Date : 2020-06-01 Feifei Feng, Li Huang, Guoyu Zhou, Jia Wang, Ruiqin Zhang, Zhiyuan Li, Yawei Zhang, Yue Ba
To explore the impact of air pollutants exposure during pregnancy on infant DNA methylation, we identified correlated methylated genes in maternal and cord blood samples using the Illumina Human Methylation 27 k BeadChip. Quantitative methylation-specific PCR (QMS-PCR) was performed to validate the target gene methylation pattern in 568 participants. Then the association between air pollutants exposure and DNA methylation level in the target gene was investigated. The GPR61 gene with a higher methylation level both in mothers and newborns was identified as the target gene, and we found a positive mother-infant DNA methylation correlation in the promoter region of GPR61. Air pollutants exposure during entire pregnancy was associated with maternal and infant GPR61 DNA methylation. After adjusting confounding variables, maternal air pollutants exposure was still associated with infant GPR61 DNA methylation. In summary, GPR61 methylation in cord blood may be a potential target of prenatal exposure to air pollutants.
中文翻译:
脐带血中的GPR61甲基化:产前暴露于空气污染物的潜在目标
为了探索怀孕期间空气污染物暴露对婴儿DNA甲基化的影响,我们使用Illumina人类甲基化27 k BeadChip在母体和脐带血样品中鉴定了相关的甲基化基因。进行了量化甲基化特异性PCR(QMS-PCR)以验证568位参与者中的靶基因甲基化模式。然后研究了空气污染物暴露与目标基因中DNA甲基化水平之间的关系。在母亲和新生儿中甲基化水平较高的GPR61基因被鉴定为靶基因,我们在GPR61的启动子区域发现了母婴DNA甲基化呈正相关。整个怀孕期间空气污染物的暴露与母婴GPR61相关DNA甲基化。调整混杂变量后,母亲空气污染物的暴露仍与婴儿GPR61 DNA甲基化有关。总之,脐带血中的GPR61甲基化可能是产前暴露于空气污染物的潜在目标。