This study investigated the antifungal activity and the potential antifungal mechanisms of 2‐hydroxy‐4‐methoxybenzaldehyde (HMB) against Aspergillus flavus (A. flavus). The minimum inhibitory concentration (MIC) of HMB in preventing spore germination was 70 μg mL⁻¹. HMB at MIC disrupted cell wall integrity by reducing the number of septa by 86.66% (P < 0.05) in mycelia and increased cell membrane permeability by about 14‐fold (P < 0.05) evidenced by propidium iodide (PI) staining. Furthermore, HMB at MIC inhibited respiration by 33.33%. These results revealed that the antifungal activity of HMB against A. flavus could be attributed to the damaged cell wall integrity, cell membrane permeability and respiration metabolism. What’s more, A. flavus was completely restrained in corn kernels due to HMB. Therefore, HMB could be applied as an effective antifungal agent.

中文翻译：

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2-羟基-4-甲氧基苯甲醛通过破坏细胞壁，细胞膜，控制呼吸作用来抑制黄曲霉的生长，从而对玉米粒产生有希望的抗真菌作用

这项研究调查了2-羟基-4-甲氧基苯甲醛（HMB）对黄曲霉（A. flavus）的抗真菌活性和潜在的抗真菌机制。防止孢子萌发的HMB最低抑菌浓度（MIC）为70μgmL ^-1。MIC处的HMB通过使 菌丝体中的隔垫数量减少86.66％（P <0.05）和使细胞膜通透性增加约14倍（P  <0.05）而破坏了细胞壁的完整性，碘化丙锭（PI）染色证明了这一点。此外，MIC处的HMB抑制了33.33％的呼吸。这些结果表明，HMB对黄曲霉具有抗真菌活性。可能归因于受损的细胞壁完整性，细胞膜通透性和呼吸代谢。此外，由于HMB ，黄曲霉被完全抑制了玉米粒。因此，HMB可以用作有效的抗真菌剂。