The characterization of degradation of biodegradable materials for tissue regeneration is classically carried out in three steps: in vitro degradation analysis, in vitro cell culture, and in vivo animal experiments. Each step involves an increasing complexity and should serve a more sophisticated material selection, which serves as an orientation to clinical studies and the final application in patients. Recently, the usefulness of degradation analyses is being discussed. In this context, the aim of this work is to increase the importance of in vitro degradation analysis by using flowing media to move closer to the in vivo situation. In the long term, this should lead to a more sensitive biomaterial characterization as well as to a replacement of time‐consuming static or quasi‐dynamic incubation experiments. The practicability of the novel chamber is demonstrated in context of a degradation study of silica/collagen/calcium phosphate composites in flowing media with physiological (2.4 mM) and lowered (0.5 mM) calcium ion concentrations. This is done by comparison with static and quasi‐dynamic incubation experiments. In order to keep all media regimes comparable to each other, for the dynamic experiment, a flow rate was chosen equivalent to the medium exchange in quasi‐dynamic incubation. Under flow‐through conditions, there is a clearly decreased tendency to lower the calcium concentration, so that a concentration close to the physiological initial situation can be continuously maintained.

中文翻译：

---

用于体外生物材料表征的新型降解流通室。

用于组织再生的可生物降解材料的降解表征通常分三个步骤进行：体外降解分析、体外细胞培养和体内动物实验。每个步骤都涉及越来越多的复杂性，应该提供更复杂的材料选择，作为临床研究和患者最终应用的方向。最近，正在讨论降解分析的有用性。在这种情况下，这项工作的目的是通过使用流动介质更接近体内情况来增加体外降解分析的重要性。从长远来看，这将导致更敏感的生物材料表征以及替代耗时的静态或准动态孵化实验。在具有生理 (2.4 mM) 和降低 (0.5 mM) 钙离子浓度的流动介质中，二氧化硅/胶原蛋白/磷酸钙复合材料的降解研究证明了新型腔室的实用性。这是通过与静态和准动态孵化实验进行比较来完成的。为了保持所有培养基状态相互比较，对于动态实验，选择与准动态孵化中培养基交换等效的流速。在流通条件下，钙浓度降低的趋势明显降低，因此可以持续保持接近生理初始状态的浓度。5 mM) 钙离子浓度。这是通过与静态和准动态孵化实验进行比较来完成的。为了保持所有培养基状态相互比较，对于动态实验，选择与准动态孵化中培养基交换等效的流速。在流通条件下，钙浓度降低的趋势明显降低，因此可以持续保持接近生理初始状态的浓度。5 mM) 钙离子浓度。这是通过与静态和准动态孵化实验进行比较来完成的。为了保持所有培养基状态相互比较，对于动态实验，选择与准动态孵化中培养基交换等效的流速。在流通条件下，钙浓度降低的趋势明显降低，因此可以持续保持接近生理初始状态的浓度。