Flowcytometric analysis of lymphocytes and their subpopulations in bronchoalveolar lavages (BAL) can support the diagnosis of interstitial lung diseases. This analysis should be done within 4 hr after lavage due to rapid cell deterioration. We tested three methods in order to stabilize for at least 28 days the BAL cell populations to allow delayed flowcytometric analysis in order to facilitate external quality assurance (EQA).

中文翻译：

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用于流式细胞仪分析的三种稳定支气管肺泡灌洗细胞方法的比较

支气管肺泡灌洗液 (BAL) 中淋巴细胞及其亚群的流式细胞术分析可以支持间质性肺疾病的诊断。由于细胞快速退化，该分析应在灌洗后 4 小时内完成。我们测试了三种方法，以使 BAL 细胞群稳定至少 28 天，以允许延迟流式细胞术分析，以促进外部质量保证 (EQA)。