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Reducing Agent-Mediated Nonenzymatic Conversion of 2-Oxoglutarate to Succinate: Implications for Oxygenase Assays.
ChemBioChem ( IF 3.2 ) Pub Date : 2020-06-01 , DOI: 10.1002/cbic.202000185
Amjad Khan 1 , Christopher J Schofield 1 , Timothy D W Claridge 1
Affiliation  

Taking everything into account: In catalytic reactions by some 2OG oxygenases with prolonged reaction times or high l‐ascorbate concentrations, nonenzymatic reactions of 2‐oxoacids mediated by reactive oxygen species such as H2O2 might be relevant. Knowledge of how catalysis is affected by, say, metal ions or temperature will allow appropriate controls to be implemented in kinetic studies or inhibition assays.
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中文翻译:

还原剂介导的2-氧戊二酸酯向琥珀酸酯的非酶促转化:对氧化酶测定的影响。

考虑到所有因素:在某些2OG加氧酶的催化反应中,如果反应时间延长或l抗坏血酸浓度较高,则由活性氧(例如H 2 O 2)介导的2-含氧酸的非酶反应可能是相关的。知道例如金属离子或温度如何影响催化作用,将允许在动力学研究或抑制试验中实施适当的控制。
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更新日期:2020-06-01
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