Abstract Submillimetric hollow fiber (HOF-) attenuated total reflection (ATR-) Fourier transform infrared spectroscopy (FTIRS) technique is very suitable for in situ detection of biological samples. HOF-ATR-FTIRS analysis discloses that the significant changes in collagen fiber and proteoglycan between healthy and osteoarthritic (OA) canine specimens. The technique combined with principal component analysis (PCA) and Fisher’s discriminant analysis (FDA) was used to further identify in situ healthy and OA cartilage specimens. The sensitivity of 100% and specificity of 93.6% were obtained for prediction group after all the cases were correctly identified for initial sample, and cross validation was identified with 100% accuracy. The HOF-ATR-FTIRS technique is convenient for operation and suitable for micro-area in situ diagnosis, while the FDA method is fast and accurate. The combination of HOF-ATR-FTIR technique and FDA method can fast realize the micro-area detection and classification of in situ samples with good accuracy, which has potential ability to provide a practical clinical diagnosis of OA.

中文翻译：

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中空纤维衰减全反射傅里叶变换红外光谱与Fisher判别分析鉴别犬类健康关节软骨和骨关节炎关节软骨的初步研究

摘要 亚毫米中空纤维（HOF-）衰减全反射（ATR-）傅里叶变换红外光谱（FTIRS）技术非常适用于生物样品的原位检测。HOF-ATR-FTIRS 分析揭示了健康和骨关节炎 (OA) 犬标本之间胶原纤维和蛋白多糖的显着变化。该技术结合主成分分析 (PCA) 和 Fisher 判别分析 (FDA) 用于进一步识别原位健康和 OA 软骨标本。初始样本正确识别所有病例后，预测组的敏感性为100%，特异性为93.6%，交叉验证的准确率为100%。HOF-ATR-FTIRS技术操作方便，适用于微区原位诊断，而 FDA 的方法又快又准确。HOF-ATR-FTIR技术与FDA方法相结合，可以快速、准确地实现原位样品的微区检测和分类，具有为OA的临床诊断提供潜在能力。