MYB/c-MYB is a proto-oncogene encoding a helix-turn-helix transcription factor that plays a critical role in controlling proliferation and multilineage differentiation of hematopoietic progenitor and stem cells. Deregulation of MYB expression is associated with several types of leukemias and lymphomas. In an attempt to explore the role of the gene in the early human hematopoiesis, we have achieved bi-allelic targeting of MYB in human embryonic stem cells (hESCs) by TALEN-mediated homologous recombination. Furthermore, the gene targeting introduced eYFP Venus reporter gene into the MYB locus to delineate the expression pattern of MYB. The resulting two cell lines, WAe001-A-45 and WAe001-A-46, passed the standard assays for human pluripotent stem cells. Hematopoietic differentiation of these cell lines provides a model to study the role of MYB in human hematopoietic development.

MYB/c-MYB是编码螺旋-转角-螺旋转录因子的原癌基因，在控制造血祖细胞和干细胞的增殖和多向分化中起关键作用。MYB表达的失调与几种类型的白血病和淋巴瘤有关。为了探索该基因在早期人类造血中的作用，我们通过 TALEN 介导的同源重组实现了人类胚胎干细胞 (hESCs)中MYB的双等位基因靶向。此外，基因靶向将 eYFP Venus报告基因引入MYB基因座以描绘 MYB 的表达模式. 所得的两种细胞系 WAe001-A-45 和 WAe001-A-46 通过了人类多能干细胞的标准检测。这些细胞系的造血分化为研究 MYB 在人类造血发育中的作用提供了一个模型。