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Human norovirus detection in bivalve shellfish in Brazil and evaluation of viral infectivity using PMA treatment.
Marine Pollution Bulletin ( IF 5.8 ) Pub Date : 2020-06-01 , DOI: 10.1016/j.marpolbul.2020.111315
Sylvia Kahwage Sarmento 1 , Caroline Rezende Guerra 2 , Fábio Correia Malta 1 , Ricardo Coutinho 2 , Marize Pereira Miagostovich 1 , Tulio Machado Fumian 1
Affiliation  

Noroviruses are the most common cause of gastroenteritis outbreaks in humans and bivalve shellfish consumption is a recognized route of infection. Our aim was to detect and characterize norovirus in bivalves from a coastal city of Brazil. Nucleic acid was extracted from the bivalve's digestive tissue concentrates using magnetic beads. From March 2018 to June 2019, 77 samples were screened using quantitative RT-PCR. Noroviruses were detected in 41.5%, with the GII being the most prevalent (37.7%). The highest viral load was 3.5 × 106 and 2.5 × 105 GC/g in oysters and mussels, respectively. PMA-treatment demonstrated that a large fraction of the detected norovirus corresponded to non-infectious particles. Genetic characterization showed the circulation of the GII.2[P16] and GII.4[P4] genotypes. Norovirus detection in bivalves reflects the anthropogenic impact on marine environment and serves as an early warning for the food-borne disease outbreaks resulting from the consumption of contaminated molluscs.



中文翻译:

在巴西双壳贝类中检测人类诺如病毒,并使用PMA处理评估病毒感染性。

诺如病毒是人类胃肠炎暴发的最常见原因,食用双壳贝类是公认的感染途径。我们的目标是检测和鉴定来自巴西沿海城市的双壳类中的诺如病毒。使用磁珠从双壳类消化组织浓缩物中提取核酸。从2018年3月到2019年6月,使用定量RT-PCR筛选了77个样品。诺如病毒的检出率为41.5%,其中GII最为流行(37.7%)。牡蛎和贻贝的最高病毒载量分别为3.5×106和2.5×105 GC / g。PMA处理表明,检测到的诺如病毒的大部分对应于非感染性颗粒。遗传特征表明GII.2 [P16]和GII.4 [P4]基因型的循环。

更新日期:2020-06-01
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