African swine fever (ASF) is an acute, hemorrhagic, highly contagious disease caused by African swine fever virus (ASFV) infection of domestic pigs and wild boars, showing mortality rates up to 100 %. There are no effective vaccines or antiviral drugs available for ASFV. Therefore, disease control is mainly based on animal slaughtering and the enforcement of strict sanitary measures. In order to establish a rapid, sensitive and simple method for on-site detection of ASFV, a recombinase polymerase amplification (RPA) combined with lateral flow dipstick (LFD) was developed using a pair of specific primers and probe. Using recombinant plasmid pMD19-T-K205R DNA as a template, the RPA-LFD detection could be accomplished in 10 min at a temperature of 36℃-44℃. More specific than PCR and more rapid and simpler than real-time PCR, RPA-LFD has the same detection limit of 1 × 10² copies/reaction as real-time PCR, also with no cross-reaction with other viral strains. A convenient and rapid ASFV RPA-LFD detection method was developed, which will provide an efficient method for investigating epidemiology of ASFV infection.

非洲猪瘟（ASF）是由非洲猪瘟病毒（ASFV）感染家猪和野猪引起的一种急性，出血性，高传染性疾病，其死亡率高达100％。没有适用于ASFV的有效疫苗或抗病毒药物。因此，疾病控制主要基于屠宰动物和执行严格的卫生措施。为了建立一种快速，灵敏和简单的ASFV现场检测方法，使用一对特异性引物和探针开发了结合侧向量油尺（LFD）的重组酶聚合酶扩增（RPA）。以重组质粒pMD19-T-K205R DNA为模板，RPA-LFD检测可以在36℃-44℃的温度下在10分钟内完成。比PCR更具体，比实时PCR更快速，更简单，实时PCR的^2个拷贝/反应，也没有与其他病毒株的交叉反应。开发了一种方便快捷的ASFV RPA-LFD检测方法，它将为调查ASFV感染的流行病学提供一种有效的方法。