Large-scale use of ammonia, sulphate, and nitrate in industrial manufacturing has resulted in the generation of industrial wastewater pollutants. However, approaches to eliminate such contamination have not been extensively studied. Accordingly, in this study, we investigated the succession of bacteria under different influent loadings in a mixotrophic denitrification desulphurisation system. Four expanded granular sludge bed reactors were operated simultaneously. The sulphide loading of reactor I was 1.2 kg/m³‧day, the sulphide load of reactor II was 2.4 kg/m³‧day, and the sulphide load of reactor III was 3.6 kg/m³‧day. The molar ratio of carbon versus nitrogen in the influent under each condition was fixed at 1.26:1, and the molar ratio of sulphur versus nitrogen was fixed at 5:6; each reactor was operated for 90 days. Reactor IV was a verification reactor. The three conditions were repeated, and each condition was operated for 90 days. Middle- and late-stage samples under each condition were sequenced using a high-throughput sequencer. Azoarcus, Thauera, Arcobacter, and Pseudomonas were the core genera of the denitrification desulphurisation system under mixotrophic conditions. The genus Azoarcus was a cornerstone genus of mixotrophic conditions, as demonstrated using the random forest model and correlation network analysis.

在工业制造中大量使用氨，硫酸盐和硝酸盐已导致产生工业废水污染物。但是，尚未广泛研究消除这种污染的方法。因此，在这项研究中，我们研究了混合营养反硝化脱硫系统中不同进水量下细菌的演替。同时运行四个膨胀的颗粒污泥床反应器。反应器的硫化物负载余为1.2千克/米³ ‧day，反应器II中的硫化物负载为2.4千克/米³ ‧day，和反应器III的硫化物负载为3.6千克/米³‧天。在每种条件下，进水中碳与氮的摩尔比固定为1.26：1，硫与氮的摩尔比固定为5：6；每个反应器运行90天。反应堆IV是验证反应堆。重复这三个条件，每个条件操作90天。使用高通量测序仪对每种条件下的中晚期样品进行测序。固氮弓，Thauera，弓形，和假单胞菌是混合营养条件下，脱硝脱硫系统的核心属。固氮菌属 如使用随机森林模型和相关网络分析所证明的，它是混养条件的基石属。