(–)-epigallocatechin-3-O-gallate (EGCG) is a bioactive polyphenol in green tea. Previous studies have demonstrated the beneficial effects of EGCG on muscle mass and muscle atrophy. In the current study, we investigated the mechanisms underlying effect of EGCG on muscle atrophy. It was demonstrated that EGCG suppressed muscle-specific ubiquitin ligase, muscle RING Finger 1 (MuRF1) expression through 67-kDa laminin receptor (67LR). Previous studies have shown that eriodictyol potentiates the anti-tumor activities of EGCG by amplifying 67LR signaling. Therefore, we investigated the effects of EGCG and eriodictyol on the MuRF1 expression in C2C12 myotubes. The combined treatment of EGCG and eriodictyol significantly suppressed MuRF1 expression in dexamethasone-treated C2C12 myotubes. Tail suspension was maintained for 10 consecutive days using C57BL6/J mice, and during this time EGCG and eriodictyol were orally administered. In the gastrocnemius muscle, the muscle mass loss was inhibited by the combination of EGCG and eriodictyol. Therefore, EGCG may prevent muscle atrophy by inducing 67LR signaling and eriodictyol amplifies this pathway.

中文翻译：

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EGCG通过67 kDa层粘连蛋白受体下调MuRF1表达，而雌三醇可放大该受体信号。

（–）-epigallocatechin-3- O没食子酸（EGCG）是绿茶中的一种生物活性多酚。先前的研究已证明EGCG对肌肉质量和肌肉萎缩的有益作用。在当前的研究中，我们调查了EGCG对肌肉萎缩的潜在作用机理。结果表明，EGCG通过67 kDa层粘连蛋白受体（67LR）抑制了肌肉特异性泛素连接酶，肌肉RING Finger 1（MuRF1）的表达。先前的研究表明，淫羊dict酚可通过放大67LR信号转导来增强EGCG的抗肿瘤活性。因此，我们调查了EGCG和Eriodictyol对C2C12肌管中MuRF1表达的影响。在地塞米松处理过的C2C12肌管中，EGCG和芥酸的联合治疗可显着抑制MuRF1表达。使用C57BL6 / J小鼠连续10天保持尾部悬吊，在这段时间里口服了EGCG和Eriodictyol。在腓肠肌中，EGCG和Erdicticol的组合可抑制肌肉质量的损失。因此，EGCG可以通过诱导67LR信号传导来预防肌肉萎缩，而雌雄醇则可以放大该途径。