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Complete genome sequence analysis of a genetic variant of grapevine virus L from the grapevine cultivar Blanc du Bois.
Archives of Virology ( IF 2.7 ) Pub Date : 2020-05-30 , DOI: 10.1007/s00705-020-04682-8
Olufemi J Alabi 1 , David N Appel 2 , Sheila McBride 2 , Maher Al Rwahnih 3 , Fran M Pontasch 4
Affiliation  

The complete genome sequences of two grapevine virus L (GVL) isolates collected from the wine grape cultivar Blanc du Bois (Vitis spp.: ‘Florida D 6-148’בCardinal’) in Texas were determined. The two genome sequences (excluding the polyA tail) were each 7594 nucleotide long and 99.7% identical to each other, but they shared only ~74% identity with those of previously published GVL isolates. Further analysis showed that the two Texas GVL isolates also diverged significantly from previously published isolates of the virus in each of the five ORFs at both the nucleotide and amino acid level, indicating that they represent a new phylogroup of this virus.



中文翻译:

来自葡萄品种Blanc du Bois的葡萄病毒L的遗传变异的完整基因组序列分析。

确定了从得克萨斯州的酿酒葡萄品种Blanc du Bois(Vitis spp .:'Florida D 6-148'×'Cardinal')收集的两种葡萄病毒L(GVL)分离株的完整基因组序列。这两个基因组序列(不包括polyA尾巴)各有7594个核苷酸长,彼此相同,占99.7%,但与以前发表的GVL分离株仅具有约74%的同一性。进一步的分析表明,两个德克萨斯州的GVL分离物在核苷酸水平和氨基酸水平上,在五个ORF的每一个中也与以前公布的病毒分离物有很大差异,表明它们代表了该病毒的一个新的系统群。

更新日期:2020-07-13
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