Abstract

The present study is the first attempt made to investigate the effects of diabetes on expression and promoter DNA methylation of TGF-β1, ESR-1, and CDH-1 genes and also the effects of folic acid (FA) and vitamin E (Vit E) supplementations on improving diabetes mellitus. STZ-induced diabetic rats were treated with Vit E (200 mg/kg/day) and FA (25 mg/kg/day) for 8 weeks and expression and DNA methylation of TGF-β1, ESR-1, and CDH-1 genes in uterus were analysed. Data indicated that diabetes increases the expression of TGFβ-1 and ESR-1 and decreases CDH-1 expression and TGFβ-1 promoter methylation in the uterus of rats. Vit E and FA improved the negative effects of diabetes by decreasing the expression of TGFβ-1 and ESR-1 and increasing that of CDH-1 in diabetic rats. In conclusion, these findings emphasise that Vit E and FA supplementations could improve negative effects caused by diabetes on uterus function and fertility in diabetic rats.

摘要

本研究首次尝试研究糖尿病对 TGF-β1、ESR-1 和 CDH-1 基因表达和启动子 DNA 甲基化的影响，以及叶酸 (FA) 和维生素 E (Vit E) 的影响。 ) 改善糖尿病的补充剂。STZ 诱导的糖尿病大鼠用 Vit E（200 mg/kg/天）和 FA（25 mg/kg/天）治疗 8 周，TGF-β1、ESR-1 和 CDH-1 基因的表达和 DNA 甲基化在子宫内进行了分析。数据表明，糖尿病增加大鼠子宫中TGFβ-1和ESR-1的表达，降低CDH-1表达和TGFβ-1启动子甲基化。Vit E 和 FA 通过降低糖尿病大鼠 TGFβ-1 和 ESR-1 的表达和增加 CDH-1 的表达来改善糖尿病的负面影响。综上所述，