Abstract

Sophora tonkinensis is widely used as traditional Chinese medicine for treating the swelling of the gums and tongue and mouth sores due to flame stomach fire. It is mainly origin from Guangxi, Sichuan provinces of China. Alkaloids are considered as the major bioactive components. A method was established for identifying alkaloids in S. tonkinensis root by UPLC-Q-TOF-MS/MS and was applied in characterizing alkaloids in S. tonkinensis root of two different habitats. Consequently, twenty-four alkaloids including six new compounds were identified in S. tonkinensis root. Additionally, the difference of alkaloids in S. tonkinensis from Guozhou, Sichuan province was investigated. In the present study, we firstly characterize total alkaloids in S. tonkinensis root by UPLC-Q-TOF-MS/MS and firstly established the characteristic fragmentation pathway of alkaloids with hydroxy in S. tonkinensis root.

摘要

山豆根被广泛用作中药，用于治疗因火焰胃火引起的牙龈肿胀和舌口疮。主要产于广西、四川等地。生物碱被认为是主要的生物活性成分。建立了UPLC-Q-TOF-MS/MS鉴定山豆根中生物碱的方法，并应用于两种不同生境的山豆根中生物碱的表征。因此，在山豆根中鉴定出 24 种生物碱，包括 6 种新化合物。此外，山豆蔻中生物碱的差异来自四川省果州的调查。本研究首次采用UPLC-Q-TOF-MS/MS对山豆根中的总生物碱进行表征，并首次建立了山豆根中含羟基生物碱的特征性裂解途径。