The connection between soil and microbes plays a critical role in soil health and quality and can be elastic with the application of soil amendments and/or crop rotations. Inappropriate management of soil and application of impermissible levels of fertilizers ruptures the overriding connection between the soil and microbes. This is currently evidenced in the degraded soils ( i.e ., saline soils of India) which are caused by modern agricultural practices. Reclamation of saline soils with a saturated package of practices and conventional breeding methods requires biological intervention. Shortfall of nutrients is one of the chief constraints for plant growth in salt-affected soils. In the present investigation, we have observed an arsenal of fifty halophilic bacteria carrying an absolute requirement of 3% NaCl for solubilizing the insoluble minerals (ZnCO 3 , ZnO, Mica and tri-calcium phosphate) under in vitro conditions; however, increasing the amount of NaCl over and above resulted in loss of solubilization capacity. Of the isolates solubilizing zinc carbonate and zinc oxide at 3% NaCl concentration, there were 29 isolates; at 10% concentration, 10 isolates were positive for the presence of zinc carbonate. At 3% NaCl concentration, HB-5 showed 23.16 mm zinc carbonate solubilization, HB-20 showed 13.3 mm Zinc oxide solubilization, and HB-7 showed 13.4 mm tri-calcium phosphate solubilization. Mica solubilization was peaked at 6% NaCl and maximum solubilization was observed in HB-27 (18.03 mm). When compared to the zinc carbonate solubilization, zinc oxide solubilization was slow to reach desired levels. Solubilization lasted for up to 9 days and ceased thereafter in all the tests. Eight elite isolates were identified as Bacillus albus , Bacillus safensis , Pseudomonas stutzeri (2), Lysinibacillus sphaericus , Staphylococcus xylosus , and Bacillus cereus (2) based on 16S rRNA analysis.

中文翻译：

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开采盐渍土以显示植物减轻胁迫的嗜盐细菌

土壤和微生物之间的联系在土壤健康和质量方面起着至关重要的作用，并且可以通过土壤改良剂和/或作物轮作具有弹性。对土壤的不当管理和施用过量的肥料会破坏土壤与微生物之间的重要联系。这在现代农业实践造成的退化土壤（即印度的盐渍土）中得到了证明。用一套饱和的做法和传统的育种方法开垦盐渍土需要生物干预。营养缺乏是受盐分影响的土壤中植物生长的主要制约因素之一。在目前的调查中，我们观察到五十种嗜盐细菌在体外条件下溶解不溶性矿物质（ZnCO 3 、ZnO、云母和磷酸三钙）的绝对需要量是 3% NaCl；然而，过度增加 NaCl 的量会导致溶解能力的丧失。在溶解碳酸锌和氧化锌浓度为 3% NaCl 的分离株中，有 29 株；在 10% 的浓度下，10 个分离株对碳酸锌的存在呈阳性。在 3% NaCl 浓度下，HB-5 表现出 23.16 mm 的碳酸锌溶解度，HB-20 表现出 13.3 mm 的氧化锌溶解度，而 HB-7 表现出 13.4 mm 的磷酸三钙溶解度。云母溶解在 6% NaCl 时达到峰值，在 HB-27 (18.03 mm) 中观察到最大溶解。与碳酸锌增溶相比，氧化锌溶解缓慢达到所需水平。溶解持续长达 9 天，然后在所有测试中停止。根据 16S rRNA 分析，鉴定出 8 个优良分离株为白色芽孢杆菌、安全芽孢杆菌、施氏假单胞菌 (2)、球形赖氨酸杆菌、木糖葡萄球菌和蜡状芽孢杆菌 (2)。