Introduction. TRPVs are a group of receptors with a channel activity predominantly permeable to Ca²⁺. This subfamily is involved in the development of gastrointestinal diseases such as ulcerative colitis (UC). The aim of the study was to characterize the gene and protein expression of the TRPV subfamily in UC patients and controls. Methods. We determined by quantitative PCR the gene expression of TRPV2, TRPV3, TRPV4, TRPV5, and TRPV6 in 45 UC patients (29 active UC and 16 remission UC) and 26 noninflamed controls. Protein expression was evaluated in 5 μm thick sections of formalin-fixed, paraffin-embedded tissue from 5 customized severe active UC patients and 5 control surgical specimens. Results. TRPV2 gene expression was increased in the control group compared with active UC and remission patients ( and , respectively). TRPV3 gene expression was significantly higher in controls than in active UC patients (). The gene expression of TRPV4 was significantly higher in colonic tissue from patients with remission UC compared with active UC patients () and controls (). TRPV5 had significantly higher mRNA levels in a control group compared with active UC patients (). The gene expression of TRPV6 was significantly higher in the colonic tissue from patients with active UC compared with the control group (). The protein expression of TRPV2 was upregulated in the mucosa and submucosa from the controls compared with the UC patients (). The protein expression of TRPV3 and TRPV4 was upregulated in all intestinal layers from the controls compared with the UC patients (). TRPV5 was upregulated in the submucosa and serosa from the controls vs. UC patients (). TRPV6 was upregulated in all intestinal layers from the UC patients vs. controls (). Conclusion. The TRPV subfamily clearly showed a differential expression in the UC patients compared with the controls, suggesting their role in the pathophysiology of UC.

中文翻译：

---

TRPV 亚家族（TRPV2、TRPV3、TRPV4、TRPV5 和 TRPV6）在溃疡性结肠炎患者中的基因和蛋白质表达。

简介。TRPVs是一组具有主要可透过Ca ²⁺的通道活性的受体。该亚科与溃疡性结肠炎 (UC) 等胃肠道疾病的发展有关。该研究的目的是描述 UC 患者和对照中 TRPV 亚家族的基因和蛋白质表达。方法。我们通过定量 PCR 测定了 45 名 UC 患者（29 名活动性 UC 和 16 名缓解期 UC）和 26 名非炎症对照中 TRPV2、TRPV3、TRPV4、TRPV5 和 TRPV6 的基因表达。在来自 5 名定制的严重活动性 UC 患者和 5 名对照手术标本的福尔马林固定、石蜡包埋组织的5 μ m 厚切片中评估蛋白质表达 。结果. 与活动性 UC 和缓解患者相比，对照组的 TRPV2 基因表达增加。和，分别）。对照组的 TRPV3 基因表达显着高于活动性 UC 患者（）。与活动性 UC 患者相比，缓解 UC 患者结肠组织中 TRPV4 的基因表达显着升高。)和控制 (）。与活动性 UC 患者相比，对照组的 TRPV5 mRNA 水平显着升高。）。与对照组相比，活动性 UC 患者结肠组织中 TRPV6 基因表达显着升高。）。与 UC 患者相比，对照组的黏膜和黏膜下层 TRPV2 的蛋白表达上调。）。与 UC 患者相比，对照组所有肠层中 TRPV3 和 TRPV4 的蛋白表达均上调。）。TRPV5 在对照组与UC 患者的黏膜下层和浆膜中上调（）。TRPV6 在 UC 患者与对照组的所有肠层中上调（）。 结论。与对照组相比，TRPV 亚家族在 UC 患者中明显表现出差异表达，表明它们在 UC 病理生理学中的作用。