The emergence of the huge number of multi-drug resistant (MDR) bacteria requires an alternative to the drugs. Silver nanoparticles (AgNPs) are a strong candidate for this due to their bactericidal properties, which can be better concluded by understanding their morphology and chemistry. The study hypothesised that AgNPs synthesised using leaves of Syzygium cumini can be used to treat locally emerging MDRs forming biofilms on indwelling medical devices. Synthesised particles were characterised by methods like UV-visible spectroscopy, X-ray powder diffraction, scanning electron microscopy, transmission electron microscopy, and Zetasizer. Fourier transform infrared spectroscopy, and high-performance liquid chromatography were used to predict phytochemicals present in the leaves. The shape of particles is revealed to be relatively spherical, with average size to be around 10-100 nm. Phenolic compounds are attributed to the formation of nanoparticles, stability analysis shows particles to be stable, and zeta potential determined the surface charge to be -20.1 mV. Biosynthesised particles are found to possess efficient antibacterial activity MDR bacteria developing biofilms in medical devices; hence, it is concluded that S. cumini based NPs can be used to develop a layer on implant-related medical devices. Toxicity evaluation against A594 cancer cells portrays AgNPs to be potential tumour reduction agents in a concentration-dependent manner.

中文翻译：

---

生物合成银纳米颗粒对抗多重耐药生物膜形成病原体的分析研究。

大量多重耐药（MDR）细菌的出现需要药物的替代品。银纳米颗粒（AgNP）因其杀菌特性而成为这一领域的有力候选者，通过了解其形态和化学性质可以更好地得出结论。该研究假设，使用蒲桃叶合成的 AgNP 可用于治疗在留置医疗器械上形成生物膜的局部出现的 MDR。通过紫外-可见光谱、X射线粉末衍射、扫描电子显微镜、透射电子显微镜和Zetasizer等方法对合成颗粒进行了表征。使用傅里叶变换红外光谱和高效液相色谱来预测叶子中存在的植物化学物质。颗粒形状相对球形，平均尺寸约为 10-100 nm。酚类化合物归因于纳米颗粒的形成，稳定性分析显示颗粒是稳定的，zeta 电位确定表面电荷为-20.1 mV。生物合成颗粒被发现具有高效的抗菌活性，耐多药耐药细菌在医疗器械中形成生物膜；因此，得出的结论是，基于 S. cumini 的 NP 可用于在植入物相关的医疗设备上开发一层。针对 A594 癌细胞的毒性评估表明，AgNP 是一种浓度依赖性的潜在肿瘤减少剂。