Textile wastewaters are usually alkali and saline, so using haloalkaliphilic bacteria can be the best option for the treatment of wastewater. This study aimed at the decolorization of textile Reactive Black 5 and Reactive Red 152 dyes using new haloalkaliphilic bacteria isolated from the textile wastewater. Among 50 strains of bacteria isolated from the effluent of Kashan textile industry, three bacterial strains, namely D1, D2 and E49, exhibited high decolorization abilities for Reactive Black 5 and Reactive Red 152 dyes. Decolorization was evaluated through spectrophotometry at maximum absorbance wavelengths of 607 and 554 nm for Reactive Black 5 and Reactive Red 152, respectively. The highest decolorization percentage was observed at a dye concentration of 50 mg L −1 . Aerobic conditions, 5% of the yeast extract and salt, 10% of peptone and glucose as nitrogen and carbon sources, respectively, and a pH range of 9–12 were considered as the optimal conditions for decolorization. The consortium of three haloalkaliphilic isolates showed a remarkable ability for decolorization of the Reactive Black 5 (87%) and Reactive Red 152 (85%) dyes. The consortium exhibited higher decolorization ability for the textile effluent, compared to individual bacterial inoculations. According to phenotypic characterization experiments and phylogenetic analyses based on comparing 16S rDNA sequence, the mentioned strains belonged to the genus Halomonas .

中文翻译：

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纺织废水中分离的新型嗜碱菌对活性黑5和活性红152偶氮染料的脱色

纺织废水通常是碱性和盐水，因此使用嗜碱菌是处理废水的最佳选择。本研究旨在使用从纺织废水中分离出的新型嗜碱细菌对纺织活性黑 5 和活性红 152 染料进行脱色。从喀山纺织工业废水中分离出的50株细菌中，D1、D2和E49三株细菌对活性黑5和活性红152染料表现出较高的脱色能力。脱色通过分光光度法在最大吸收波长 607 和 554 nm 下分别对活性黑 5 和活性红 152 进行评估。在50mg L -1 的染料浓度下观察到最高的脱色百分比。有氧条件下，5%的酵母提取物和盐，10% 的蛋白胨和葡萄糖分别作为氮源和碳源，pH 值范围为 9-12 被认为是脱色的最佳条件。三个卤代亲碱性分离物的联合体对活性黑 5 (87%) 和活性红 152 (85%) 染料显示出显着的脱色能力。与单个细菌接种相比，该联合体对纺织废水表现出更高的脱色能力。根据表型表征实验和基于16S rDNA序列比较的系统发育分析，上述菌株属于盐单胞菌属。三个卤代亲碱性分离物的联合体显示出显着的活性黑 5 (87%) 和活性红 152 (85%) 染料脱色能力。与单个细菌接种相比，该联合体对纺织废水表现出更高的脱色能力。根据表型表征实验和基于16S rDNA序列比较的系统发育分析，上述菌株属于盐单胞菌属。三个卤代亲碱性分离物的联合体显示出显着的活性黑 5 (87%) 和活性红 152 (85%) 染料脱色能力。与单个细菌接种相比，该联合体对纺织废水表现出更高的脱色能力。根据表型表征实验和基于16S rDNA序列比较的系统发育分析，上述菌株属于盐单胞菌属。