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Can Toxicities Induced by Insecticide Methomyl be Remediated Via Soil Bacteria Ochrobactrum thiophenivorans and Sphingomonas melonis?
Current Microbiology ( IF 2.6 ) Pub Date : 2020-05-27 , DOI: 10.1007/s00284-020-02042-y
Sule Tatar 1 , Nuran Cikcikoglu Yildirim 2 , Osman Serdar 3 , Gokhan Onder Erguven 1
Affiliation  

The research study was about revealing the biochemical response of Gammarus pulex related to insecticide methomyl before and after bioremediation by two soil bacteria species, Ochrobactrum thiophenivorans and Sphingomonas melonis. Catalase (CAT), glutathione S-transferase.(GST), cytochrome. P4501A1 (CYP1A1) activities in G. Pulex related to methomyl solution were investigated in 24 h and 96 h. ELISA method was used for test studies. CAT enzyme was decreased in Gammarus pulex that was exposed to methomyl after all exposure period (P < 0.05). CAT activities were returned to control results after bioremediation assays. GST enzyme activity was decreased depending on methomyl exposure during 24 h but increased during 4 days (P < 0.05). After 8 days of bioremediation period, GST activity increased again during 24 h while decreased during 4 days (P < 0.05). CYP1A1 activity increased in Gammarus pulex that was exposed to methomyl after all exposure period (P > 0.05). After bioremediation, statistically significant changes were not revealed in CYP1A1 activities (P > 0.05). According to the results of our study, CYP1A1, CAT, and GST activities in G. pulex sanctioned the capability of Ochrobactrum thiophenivorans and Sphingomonas melonis in methomyl bioremediation. Isolated and enriched Ochrobactrum thiophenivorans and Sphingomonas melonis that were added to 2.5 ppb concentrations of methomyl for 8 days. Each day, chemical oxygen demand (COD) and biochemical oxygen demand (BOD5), pH and dissolved oxygen parameters were monitored. At the final phase of the bioremediation step, it was determined that these bacteria have efficient methomyl bioremediation properties in a mixed corsortia at a rate of 86%. These results show that these bacteria can be used for bioremediate the receiving environments that are polluted by these kinds of insecticides.

中文翻译:

灿毒性诱发杀虫剂灭多威进行修复通过土壤细菌苍白杆菌thiophenivorans和鞘枯病?

研究性学习是关于揭示蚤有关杀虫剂灭多威的钩虾的生化反应前后两个土壤中的细菌种类,苍白杆菌thiophenivorans和鞘枯病生物修复后。过氧化氢酶(CAT),谷胱甘肽S-转移酶(GST),细胞色素。在G.蚤P4501A1(CYP1A1)的活动相关的灭多威溶液在24小时和96小时进行了调查。ELISA方法用于测试研究。在所有暴露期后暴露于灭多威的 Gammarus pulex 中 CAT 酶降低(P < 0.05)。CAT活性生物修复试验后恢复控制的结果。取决于灭多威曝光GST酶的活性降低24小时内,但在4天(P <0.05)增加。生物修复后,为期8天,而在4天(P <0.05)降低的GST活性24小时期间再次增加。CYP1A1活性这是所有的曝光周期(P> 0.05)后暴露于灭多威钩虾状溞增加。生物修复后,统计学显著变化均未CYP1A1活性(P> 0.05)显露。根据我们的研究,CYP1A1,CAT和GST活动G.结果蚤认可的人苍白杆菌thiophenivorans的能力和鞘氨醇中灭多威生物修复枯病。分离和富集加入灭多威2.5 ppb的浓度8天的是苍白杆菌thiophenivorans和鞘氨醇单枯病。每天监测化学需氧量 (COD) 和生化需氧量 (BOD5)、pH 值和溶解氧参数。在生物修复步骤的最后阶段,它被确定这些细菌在86%的速度在混合corsortia高效灭多虫生物修复性质。这些结果表明,这些细菌可用于生物修复被这类杀虫剂污染的接收环境。
更新日期:2020-05-27
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