The direct electron transfer between human cytoglobin (Cygb) and the electrode surface, which would allow manipulating the oxidation states of the heme iron in Cygb, was first observed by immobilizing Cygb on a nanoporous gold (NPG) electrode via a carboxy-terminated alkanethiol. The voltammetric performances of the wild type and mutated Cygb-immobilized NPG electrodes were evaluated in the absence or presence of potential substrates. The obtained results demonstrated that the usefulness of the proposed method in understanding the function of Cygb in molecular basis.

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中文翻译：

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通过直接电化学方法研究人类细胞色素的氧化还原状态，以研究其在分子基础上的功能。

首先通过经由羧基端基的链烷硫醇将Cygb固定在纳米多孔金（NPG）电极上，从而观察到人血红蛋白（Cygb）与电极表面之间的直接电子转移，该转移可以操纵Cygb中血红素铁的氧化态。在没有或没有潜在底物的情况下，评估了野生型和突变的Cygb固定NPG电极的伏安性能。所得结果表明，所提出的方法在理解Cygb的分子功能方面是有用的。

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