当前位置: X-MOL 学术Cells Tissues Organs › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Neuropathic Pain Causes a Decrease in the Dendritic Tree Complexity of Hippocampal CA3 Pyramidal Neurons
Cells Tissues Organs ( IF 2.7 ) Pub Date : 2019-01-01 , DOI: 10.1159/000506812
Anna A Tyrtyshnaia 1 , Igor V Manzhulo 2 , Sophia P Konovalova 2 , Anna A Zagliadkina 2
Affiliation  

The International Pain Association defines neuropathic pain as “an unpleasant sensory and emotional experience associated with actual or potential tissue damage.” Recent studies show that chronic neuropathic pain causes both morphological and functional changes within brain structures. Due to the impact of supraspinal centers on pain signal processing, patients with chronic pain often suffer from depression, anxiety, memory impairment, and learning disabilities. Changes in hippocampal neuronal and glial plasticity can play a substantial role in the development of these symptoms. Given the special role of the CA3 hippocampal area in chronic stress reactions, we suggested that this region may undergo significant morphological changes as a result of persistent pain. Since the CA3 area is involved in the implementation of hippocampus-dependent memory, changes in the neuronal morphology can cause cognitive impairment observed in chronic neuropathic pain. This study aimed to elucidate the structural and plastic changes within the hippocampus associated with dendritic tree atrophy of CA3 pyramidal neurons in mice with chronic sciatic nerve constriction. Behavioral testing revealed impaired working and long-term memory in mice with a chronic constriction injury. Using the Golgi-Cox method, we revealed a decrease in the number of branches and dendritic length of CA3 pyramidal neurons. The dendritic spine number was decreased, predominantly due to a reduction in mushroom spines. An immunohistochemical study showed changes in astro- and microglial activity, which could affect the morphology of neurons both directly and indirectly via the regulation of neurotrophic factor synthesis. Using ELISA, we found a decrease in brain-derived neurotrophic factor production and an increase in neurotrophin-3 production. Morphological and biochemical changes in the CA3 area are accompanied by impaired working and long-term memory of animals. Thus, we can conclude that morphological and biochemical changes within the CA3 hippocampal area may underlie the cognitive impairment in neuropathic pain.

中文翻译:

神经性疼痛导致海马 CA3 锥体神经元树突树复杂性降低

国际疼痛协会将神经性疼痛定义为“与实际或潜在组织损伤相关的不愉快的感觉和情绪体验”。最近的研究表明,慢性神经性疼痛会导致大脑结构的形态和功能发生变化。由于脊上中枢对疼痛信号处理的影响,慢性疼痛患者经常出现抑郁、焦虑、记忆障碍和学习障碍。海马神经元和神经胶质可塑性的变化可以在这些症状的发展中发挥重要作用。鉴于 CA3 海马区在慢性应激反应中的特殊作用,我们认为该区域可能由于持续性疼痛而发生显着的形态变化。由于CA3区参与了海马依赖性记忆的实现,在慢性神经性疼痛中观察到的神经元形态的变化可引起认知障碍。本研究旨在阐明与慢性坐骨神经收缩小鼠 CA3 锥体神经元树突状树萎缩相关的海马结构和可塑性变化。行为测试显示患有慢性收缩损伤的小鼠的工作和长期记忆受损。使用 Golgi-Cox 方法,我们发现 CA3 锥体神经元的分支数量和树突长度减少。树突棘数量减少,主要是由于蘑菇棘减少。一项免疫组织化学研究表明,星形胶质细胞和小胶质细胞活性的变化可以通过调节神经营养因子合成直接和间接影响神经元的形态。使用ELISA,我们发现脑源性神经营养因子的产生减少,而神经营养因子-3 的产生增加。CA3 区域的形态和生化变化伴随着动物的工作和长期记忆受损。因此,我们可以得出结论,CA3 海马区的形态学和生化变化可能是神经性疼痛认知障碍的基础。
更新日期:2019-01-01
down
wechat
bug