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Novel enteric viruses in fatal enteritis of grey squirrels.
Journal of General Virology ( IF 3.8 ) Pub Date : 2020-07-01 , DOI: 10.1099/jgv.0.001431
Akbar Dastjerdi 1 , Camilla Benfield 2 , David Everest 1 , Mark F Stidworthy 3 , Roland Zell 4
Affiliation  

Astro- and kobuviruses infect both humans and animals. Here, we report on the disease history, detection and genomic characterization of novel astro- and kobuviruses from fatal diarrhoea of two juvenile grey squirrels. The virus particles had enterovirus-like morphology and a diameter of 28–32 nm. Next-generation sequencing confirmed astro- and kobuviruses and sequence analysis revealed typical astrovirus and picornavirus genome organizations. The astrovirus ORF2 sequence clustered with a clade of unassigned astroviruses, with marmot and rodent mamastroviruses as closest relatives. For the kobuvirus, divergences greater than 49.4 % for P1 and 43.5 % in the non-structural proteins indicated a novel species. However, phylogenetic analysis of the 3D polymerase showed that it clustered with that of the newly classified ludopivirus A1, suggesting a previous recombination event in the evolution of the kobuvirus. Our data provide further insights into the diversity of astro- and kobuviruses and broaden the spectrum of viruses infecting grey squirrels.

中文翻译:

新型肠道病毒在灰松鼠的致命性肠炎中。

星形和科布病毒同时感染人类和动物。在这里,我们从两只幼年灰松鼠的致命性腹泻报告了新的星状病毒和科布病毒的病史,检测和基因组特征。病毒颗粒具有肠病毒样形态,直径为28-32 nm。下一代测序证实了星状病毒和甲型肝炎病毒,序列分析显示了典型的星状病毒和小核糖核酸病毒基因组结构。星状病毒ORF2序列聚集了一大堆未分配的星状病毒,其中土拨鼠和啮齿类哺乳动物病毒为近亲。对于Kobuvirus,P1的差异大于49.4%,非结构蛋白的差异大于43.5%,表明是一种新物种。但是,对3D聚合酶的系统发育分析表明,它与新分类的ludopivirus A1聚在一起,提示在甲壳病毒进化过程中发生了先前的重组事件。我们的数据提供了对星状和科布病毒多样性的进一步了解,并扩大了感染灰松鼠的病毒范围。
更新日期:2020-08-20
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