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Simple differentiation of Salmonella Typhi, Paratyphi and Choleraesuis from Salmonella species using the eazyplex TyphiTyper LAMP assay.
Journal of Medical Microbiology ( IF 3 ) Pub Date : 2020-06-01 , DOI: 10.1099/jmm.0.001201
Jürgen Rödel 1 , Birgit Edel 1 , Sascha D Braun 2 , Ralf Ehricht 2, 3 , Sandra Simon 4 , Angelika Fruth 4 , Bettina Löffler 1
Affiliation  

Introduction. Identification of typhoidal Salmonella (TS) serovars and their discrimination from non-typhoidal Salmonella (NTS) is conventionally performed by seroagglutination. This method is labour-intensive, requires technical experience and can be inconclusive in some cases. Molecular assays may be reliable alternative diagnostic tools. Aim. This study was designed to evaluate the eazyplex TyphiTyper based on loop-mediated isothermal amplification (LAMP) for fast identification of TS and S. Choleraesuis in culture. Methodology. A total of 121 Salmonella strains and 33 isolates of other Enterobacterales species were tested by the eazyplex TyphiTyper. Simulated and clinical blood cultures (BCs) were used to examine the performance of the assay for diagnosis of systemic infection. Sample preparation took about 5 min and the test running time was 20 min. Amplification was measured by real-time fluorescence detection. Results. All TS and S. Choleraesuis strains were correctly identified. The most common NTS S. Typhimurium (n=34) and S. Enteritidis (n=15) were detected as Salmonella species without any false positive result for TS targets. Cross-reactions of NTS with TS were only rarely observed. Direct testing of positive BCs gave correct results. Sensitivities and specificities of the assay were as follows: 100 and 99.3 % for S. Typhi, 100 and 98.7 % for S. Paratyphi A, 100 and 97.3 % for S. Paratyphi B, 100 and 100 % for S. Paratyphi C, 100 and 100 % for S. Choleraesuis, and 100 and 100 % for Salmonella species, respectively. Conclusion. The eazyplex TyphiTyper is very easy to perform and allows the rapid identification of TS and S. Choleraesuis isolates.

中文翻译:

使用eazyplex TyphiTyper LAMP分析法可以从沙门氏菌中简单区分出伤寒沙门氏菌,副伤寒和霍乱沙门氏菌。

引言伤寒沙门氏菌(TS)血清型的鉴定及其与非伤寒沙门氏菌(NTS)的区别通常通过血清凝集进行。这种方法是劳动密集型的,需要技术经验,并且在某些情况下可能无法定论。分子测定法可能是可靠的替代诊断工具。目的。这项研究的目的是评估基于环介导等温扩增(LAMP)的TS和快速识别eazyplex TyphiTyper小号。霍乱文化。方法论。总共121株沙门氏菌和33株其他肠杆菌 eazyplex TyphiTyper测试了这些物种。使用模拟和临床血液培养(BCs)来检查用于诊断系统性感染的检测方法的性能。样品准备大约需要5分钟,测试运行时间为20分钟。通过实时荧光检测测量扩增。结果。所有TS和小号。霍乱弧菌菌株已正确鉴定。最常见的NTS小号。鼠伤寒沙门氏菌(Ñ = 34)和小号。肠炎沙门氏菌(n = 15)被检测为沙门氏菌 种对TS目标没有任何假阳性结果。仅很少观察到NTS与TS的交叉反应。阳性BC的直接测试给出了正确的结果。该测定法的灵敏度和特异性如下:S为100和99.3%。伤寒,S为100和98.7%。副伤寒A,S为100和97.3%。副伤寒B,S为100和100%。副伤寒C,S为100和100%。霍乱沙门氏菌沙门氏菌分别为100%和100%。结论。所述eazyplex TyphiTyper很容易进行,并允许TS和快速鉴定小号。霍乱弧菌分离物。
更新日期:2020-06-01
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