A growing body of evidence has indicated that behaviors of cancers are defined by not only intrinsic activities of tumor cells but also tumor-infiltrating immune cells (TIICs) in the tumor microenvironment. However, it still lacks a well-structured and comprehensive analysis of TIICs and its therapeutic value in esophageal cancer (EC). The proportions of 22 TIICs were evaluated between 150 normal tissues and 141 tumor tissues of EC by the CIBERSORT algorithm. Besides, correlation analyses between proportions of TIICs and clinicopathological characters, including age, gender, histologic grade, tumor location, histologic type, LRP1B mutation, TP53 mutation, tumor stage, lymph node stage, and TNM stage, were conducted. We constructed a risk score model to improve prognostic capacity with 5 TIICs by least absolute shrinkage and selection operator (lasso) regression analysis. The (immune cells in the risk model mean the proportions of immune cell infiltration in EC). Patients in the high-risk group had significantly worse overall survival than these in the low-risk group (HR: 2.146, 95% CI: 1.243-3.705, ). Finally, we identified Semustine and Sirolimus as two candidate compounds for the treatment of EC based on CMap analysis. In conclusion, the proportions of TIICs may be important to the progression, prognosis, and treatment of EC.

中文翻译：

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食管癌肿瘤浸润性免疫细胞的综合分析及相关治疗策略。

越来越多的证据表明，癌症的行为不仅由肿瘤细胞的内在活性决定，而且还由肿瘤微环境中的肿瘤浸润免疫细胞（TIIC）决定。但是，它仍然缺乏对TIICs及其在食管癌（EC）中的治疗价值的结构化和全面的分析。通过CIBERSORT算法评估了150例EC正常组织和141例EC肿瘤组织之间22种TIIC的比例。此外，对TIICs的比例与临床病理特征之间的相关性进行了分析，包括年龄，性别，组织学分级，肿瘤位置，组织学类型，LRP1B突变，TP53突变，肿瘤分期，淋巴结分期和TNM分期。我们通过最小绝对收缩和选择算子（套索）回归分析构建了一个风险评分模型，以提高5种TIIC的预后能力。的（风险模型中的免疫细胞是指EC中免疫细胞浸润的比例）。高风险组的患者总生存率明显低于低风险组（HR：2.146，95％CI：1.243-3.705，）。最后，基于CMap分析，我们确定了Semustine和Sirolimus是治疗EC的两种候选化合物。总之，TIICs的比例可能对EC的进展，预后和治疗很重要。