Combinatorial Chemistry & High Throughput Screening ( IF 1.8 ) Pub Date : 2020-12-31 , DOI: 10.2174/1386207323666200526134923 Ikenna Kingsley Uchendu 1 , Henshaw Uchechi Okoroiwu 2
Aim and Objective: Cells and tissues of the body are prone to oxidative damage as a result of an increased level of reactive oxygen species and nitrogen radical beyond the detoxifying ability of the endogenous antioxidant system. This study aimed to evaluate the ameliorative effect of methanolic extracts of Nigella sativa (MENS) against cadmium-induced blood oxidative stress and testicular toxicity in albino rats.
Materials and Methods: Twenty-five (25) male albino rats, weighing (200 ± 20g), were randomly grouped into five groups (A-E). Group B (Negative Control) received intraperitoneal administration of cadmium chloride (CdCl2, 5 mg/kg) only, group C received CdCl2 and low dose MENS (300 mg/kg, oral), group D received CdCl2 and high dose MENS (600 mg/kg, oral), group E (Positive control) received CdCl2 and Vitamin C (200 mg/kg, oral), for 14 days. No treatment was administered to group A (Normal control). The oxidative state of the blood was assessed by measuring the blood levels or activities of MDA, CAT, GSH and SOD; while testicular injury was assessed by measuring serum testosterone level using ELISA. The testes were harvested for histopathological examination.
Results: The results showed that cadmium induced a marked elevation in the level of MDA, and a decrease in SOD, CAT and GSH levels or activities (p<0.05 or p<0.01); but no significant alteration in the serum testosterone level was found (p>0.05); Histopathological studies on the testes showed that cadmium significantly induced testicular injury, which was however ameliorated by the seed extract of N. sativa.
Conclusion: We conclude that N. sativa seed extract is potentially testiculoprotective and attenuates oxidative stress against harmful chemical toxins such as cadmium.
中文翻译：
白化大鼠亚急性接触镉后血液氧化/抗氧化变化和睾丸毒性的评估：黑种草种子提取物的治疗效果。
目的和目的：由于活性氧和氮自由基的水平增加，超出了内源性抗氧化系统的解毒能力，身体的细胞和组织容易受到氧化损伤。本研究旨在评估黑种草 (MENS) 的甲醇提取物对白化大鼠中镉诱导的血液氧化应激和睾丸毒性的改善作用。
材料和方法：二十五 (25) 只雄性白化大鼠，体重 (200 ± 20g)，随机分为五组 (AE)。B组（阴性对照）仅接受氯化镉（CdCl 2，5 mg/kg）腹腔给药，C组接受CdCl 2和低剂量MENS（300 mg/kg，口服），D组接受CdCl 2和高剂量MENS （600 mg/kg，口服），E 组（阳性对照）接受 CdCl 2和维生素 C（200 毫克/千克，口服），持续 14 天。A组（正常对照）不接受任何治疗。通过测量MDA、CAT、GSH和SOD的血液水平或活性来评估血液的氧化状态；而睾丸损伤是通过使用ELISA测量血清睾酮水平来评估的。收集睾丸用于组织病理学检查。
结果：结果表明，镉引起MDA水平显着升高，SOD、CAT和GSH水平或活性降低（p<0.05或p<0.01）；但未发现血清睾酮水平有显着变化（p>0.05）；睾丸的组织病理学研究表明，镉显着诱导睾丸损伤，然而，N. sativa 的种子提取物可以改善这种损伤。
结论：我们得出结论，N. sativa 种子提取物具有潜在的睾丸保护作用，并能减轻对有害化学毒素（如镉）的氧化应激。
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