Abstract

Background: Prostate cancer (PC) is one of the most prevalent types of malignancies in males. Here, we replaced the miRNA-143 in PC cells by using a vector-based miRNA-143 transfection approach.

Materials and methods: The miRNA-143 vector was transfected into the cells and qRT-PCR was applied to assess the expression of target genes in PC3 cells. Also, the MTT, scratch wound-healing, and DAPI staining assays were done to assess the proliferation, migration, and apoptosis of the cells, respectively.

Results: The findings of the qRT-PCR determined the enhanced expression of miRNA-143 and other cancer-associated genes. The MTT and wound-healing assays revealed the proliferation and migration reduction in the transfected cells in comparison to control cells that contain an empty vector.

Conclusion: The miRNA-143 has a significant impact on cell growth and migration during PC metastasis, and it may be a promising candidate for molecular therapies of PC.

摘要

背景：前列腺癌（PC）是男性最常见的恶性肿瘤之一。在这里，我们通过使用基于载体的 miRNA-143 转染方法替换了 PC 细胞中的 miRNA-143。

材料与方法：将miRNA-143载体转染到细胞中，并应用qRT-PCR评估PC3细胞中靶基因的表达。此外，还进行了 MTT、划痕愈合和 DAPI 染色测定，以分别评估细胞的增殖、迁移和凋亡。

结果： qRT-PCR 的结果确定了 miRNA-143 和其他癌症相关基因的表达增强。MTT 和伤口愈合试验显示，与含有空载体的对照细胞相比，转染细胞的增殖和迁移减少。

结论： miRNA-143对PC转移过程中的细胞生长和迁移有显着影响，有望成为PC分子治疗的候选药物。