In Arabidopsis thaliana, a hypersensitive-like response (HR-like) is triggered underneath the eggs of the large white butterfly Pieris brassicae, and this response is dependent on salicylic acid (SA) accumulation and signaling. Previous reports indicate that the clade I L-type lectin receptor kinase LecRK-I.8 is involved in early steps of egg recognition. A genome-wide association study (GWAS) was used to better characterize the genetic structure of HR-like and discover loci that contribute to this response. We report here the identification of LecRK-I.1, a close homolog of LecRK-I.8, and show that two main haplotypes that explain part of the variation in HR-like segregate amongst natural Arabidopsis accessions. In addition, signatures of balancing selection at this locus suggest that it may be ecologically important. Disruption of LecRK-I.1 resulted in decreased HR-like and SA signaling, indicating that this protein is important for the observed responses. Furthermore, we provide evidence that LecRK-I.1 functions in the same signaling pathway as LecRK-I.8. Altogether, our results show that the response to eggs of P. brassicae is controlled by LecRKs that operate at various steps of the signaling pathway.

中文翻译：

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昆虫卵诱导的细胞死亡中的自然变异揭示了拟南芥中L型LECTIN受体KINASE-1的作用

在拟南芥中，在大型白色蝴蝶皮菜芸苔的卵下触发了类似过敏的反应（类似HR）。，并且此响应取决于水杨酸（SA）的积累和信号传导。先前的报道表明进化枝IL型凝集素受体激酶LecRK-1.8参与了卵识别的早期步骤。全基因组关联研究（GWAS）用于更好地表征HR样的遗传结构，并发现有助于此反应的基因座。我们在这里报告LecRK-I.1的鉴定，LecRK-I.8的近端同源物，并显示两个主要的单倍型，它们解释了HR样变异的一部分在天然拟南芥属种之间的分离。另外，在该基因座处进行平衡选择的标志表明它在生态上可能很重要。LecRK-1.1的中断导致减少的HR样和SA信号转导，表明该蛋白对于观察到的反应很重要。此外，我们提供的证据表明LecRK-1.1在与LecRK-1.8相同的信号通路中起作用。总之，我们的结果表明，对卵的反应芸苔假单胞菌由在信号传导途径的各个步骤中运行的LecRK控制。