Extended-spectrum beta-lactamase (ESBL) producing Enterobacteriaceae has become a major threat globally. Here we have characterized ESBL producing E. coli and K. pneumoniae from various sources, studied antibiogram and resistance gene profiles. Out of 385 samples, 31 (8.05%) were positive for ESBL producing E. coli. Such isolates could be recovered from 10.05, 8.33, 15.63, 6.67 and 4.35 per cent of cattle milk, curd, chicken, pork and cattle faeces samples, respectively. A total of 59 (15.32%) samples were positive for ESBL producing K. pneumoniae, which were isolated from 14.35, 6.25, 21.43 and 34.78 per cent cattle milk, chicken, beef and cattle faeces, respectively. All the 90 isolates were confirmed as ESBL producers by CDT and ESBL-E strip tests. Antibiogram revealed that 74.19% and 69.49% of the ESBL producing E. coli and K. pneumoniae isolates, respectively showed resistance to ceftizoxime, 25.81% and 23.73% to both co-trimoxazole and tetracycline, 19.35% and 25.42% to ciprofloxacin, 9.68% and 16.95% to chloramphenicol, 3.23% and 5.08% to pipercillin-tazobactam, and 3.23% and 3.39% to gentamicin. Resistance gene profiling showed blaCTX-M gene as most predominant (100%). The blaTEM gene was found in 54.84% and 55.93%, blaSHV gene in 90.32% and 77.97%, Sul 1 gene in 90.32% and 86.44% of ESBL producing E. coli and K. pneumoniae isolates, respectively. The Int1 gene was detected in 70.97% and 62.71% isolates, while qnrB gene was found in 3.23% and 10.17% of E. coli and K. pneumoniae isolates, respectively.

中文翻译：

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从印度阿萨姆邦的各种动物来源样品中分离出产超广谱β-内酰胺酶的大肠杆菌和肺炎克雷伯菌的表型和分子特征

产肠杆菌科细菌的广谱β-内酰胺酶（ESBL）已成为全球的主要威胁。在这里，我们对来自各种来源的产ESBL的大肠杆菌和肺炎克雷伯菌进行了表征，研究了抗菌素谱图和耐药基因图谱。在385个样本中，有31个（8.05％）产生ESBL的大肠杆菌呈阳性。可以从10.05％，8.33％，15.63％，6.67％和4.35％的牛乳，凝乳，鸡肉，猪肉和牛粪便样品中分离出此类分离物。总共59份（15.32％）样本的ESBL产肺炎克雷伯菌呈阳性，分别从14.5％，6.25％，21.43％和34.78％的牛乳，鸡肉，牛肉和牛粪中分离出来。通过CDT和ESBL-E条测试，所有90个分离株均被确认为ESBL生产者。抗菌谱显示，有74.19％和69.49％的ESBL产生大肠杆菌和K。肺炎分离株分别显示出对头孢唑肟的耐药性，对三甲恶唑和四环素的耐药率分别为25.81％和23.73％，对环丙沙星的耐药率分别为19.35％和25.42％，对氯霉素的耐药率分别为9.68％和16.95％，对哌咯西林-他唑巴坦的耐药率分别为3.23％和5.08％。 ％和3.39％的庆大霉素。抗性基因分析显示blaCTX-M基因为最主要的基因（100％）。在生产ESBL的大肠杆菌和肺炎克雷伯菌中，分别发现blaTEM基因占54.84％和55.93％，blaSHV基因占90.32％和77.97％，Sul 1基因占90.32％和86.44％。在70.97％和62.71％的分离物中检测到Int1基因，而分别在大肠杆菌和肺炎克雷伯菌中分离到的qnrB基因分别占3.23％和10.17％。氯霉素占95％，哌西林-他唑巴坦占3.23％和5.08％，庆大霉素占3.23％和3.39％。抗性基因分析显示blaCTX-M基因为最主要的基因（100％）。在生产ESBL的大肠杆菌和肺炎克雷伯菌中，分别发现blaTEM基因占54.84％和55.93％，blaSHV基因占90.32％和77.97％，Sul 1基因占90.32％和86.44％。在70.97％和62.71％的分离物中检测到Int1基因，而分别在大肠杆菌和肺炎克雷伯菌中分离到的qnrB基因分别占3.23％和10.17％。氯霉素占95％，哌西林-他唑巴坦占3.23％和5.08％，庆大霉素占3.23％和3.39％。抗性基因分析显示blaCTX-M基因为最主要的基因（100％）。在生产ESBL的大肠杆菌和肺炎克雷伯菌中，分别发现blaTEM基因占54.84％和55.93％，blaSHV基因占90.32％和77.97％，Sul 1基因占90.32％和86.44％。在70.97％和62.71％的分离物中检测到Int1基因，而分别在大肠杆菌和肺炎克雷伯菌中分离到的qnrB基因分别占3.23％和10.17％。分别。在70.97％和62.71％的分离物中检测到Int1基因，而分别在大肠杆菌和肺炎克雷伯菌中分离到的qnrB基因分别占3.23％和10.17％。分别。在70.97％和62.71％的分离物中检测到Int1基因，而分别在大肠杆菌和肺炎克雷伯菌中分离到的qnrB基因分别占3.23％和10.17％。