The transcription factor MYB15 plays an important role in grape basal immunity, and its promoter can be used as a potential target in resistance breeding. However, the regulatory mechanisms of cis-elements in its promoter region under a variety of stresses remain unclear. In this study, we identified some putative cis-regulatory elements present upstream of MYB15 in Vitis quinquangularis Shanyang (pVqMYB15_SY) and subsequently characterized the function of these elements using reporter assays. Our results showed that TCA-elements 1 and 2, ABRE, MYC and 3-AF1 binding site 1 are key cis-regulatory elements in pVqMYB15_SY and play important roles in plant bio/abiotic stress resistance.

转录因子MYB15在葡萄基础免疫中起重要作用，其启动子可用作抗性育种的潜在靶标。然而，在多种胁迫下其启动子区域中顺式元件的调控机制仍不清楚。在这项研究中，我们确定了一些假定的顺式调控元件存在于五角山葡萄（MYV15MY）的MYB15上游（pVqMYB15 _SY），随后使用报告基因分析法表征了这些元件的功能。我们的结果表明，TCA元素1和2，ABRE，MYC和3-AF1结合位点1是pVqMYB15中关键的顺式调控元件_SY并在植物生物/非生物逆境抗性中发挥重要作用。