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Τhe Nematicidal Potential of Bioactive Streptomyces Strains Isolated from Greek Rhizosphere Soils Tested on Arabidopsis Plants of Varying Susceptibility to Meloidogyne spp.
Plants ( IF 4.658 ) Pub Date : 2020-05-30 , DOI: 10.3390/plants9060699
Christianna Meidani 1 , Alexandros Savvidis 1 , Evaggelia Lampropoulou 1 , Aggeliki Sagia 1 , Efstathios Katsifas 1 , Nikolaos Monokrousos 2 , Dimitris G Hatzinikolaou 1 , Amalia D Karagouni 1 , Eleni Giannoutsou 1 , Ioannis-Dimosthenis S Adamakis 1 , Nikoletta G Ntalli 3
Affiliation  

A total of 461 indigenous Streptomycetes strains recovered from various Greek rhizosphere habitats were tested for their bioactivity. All isolates were examined for their ability to suppress the growth of 12 specific target microorganisms. Twenty-six were found to exert antimicrobial activity and were screened for potential nematicidal action. S. monomycini ATHUBA 220, S. colombiensis ATHUBA 438, S. colombiensis ATHUBA 431, and S. youssoufensis ATHUBA 546 were proved to have a nematicidal effect and thus were further sequenced. Batch culture supernatants and solvent extracts were assessed for paralysis on Meloidogyne javanica and Meloidogyne incognita second-stage juveniles (J2). The solvent extracts of S. monomycini ATHUBA 220 and S. colombiensis ATHUBA 438 had the highest paralysis rates, so these Streptomycetes strains were further on tested for nematodes’ biological cycle arrest on two Arabidopsis thaliana plants; the wild type (Col-0) and the katanin mutant fra2, which is susceptible to M. incognita. Interestingly, S. monomycini ATHUBA 220 and S. colombiensis ATHUBA 438 were able to negatively affect the M. incognita biological cycle in Col-0 and fra2 respectively, and increased growth in Col-0 upon M. incognita infection. However, they were ineffective against M. javanica. Fra2 plants were also proved susceptible to M. javanica infestation, with a reduced growth upon treatments with the Streptomyces strains. The nematicidal action and the plant-growth modulating abilities of the selected Streptomycetes strains are discussed.

中文翻译:

从希腊根际土壤中分离的生物活性链霉菌菌株的杀线虫潜力,在对根结线虫属不同敏感性的拟南芥植物上进行了测试。

对从希腊不同根际栖息地回收的总共 461 种本土链霉菌菌株进行了生物活性测试。检查所有分离株抑制 12 种特定目标微生物生长的能力。发现 26 种具有抗菌活性,并对其潜在的杀线虫作用进行了筛选。单霉素S. monomycini ATHUBA 220、S. colombiensis ATHUBA 438、S. colombiensis ATHUBA 431和S. youssoufensis ATHUBA 546被证明具有杀线虫作用,因此被进一步测序。评估分批培养物上清液和溶剂提取物对爪哇根结线虫南方根结线虫第二阶段幼虫(J2)的麻痹情况。S. monomycini ATHUBA 220 和S. colombiensis ATHUBA 438的溶剂提取物具有最高的麻痹率,因此进一步测试了这些链霉菌菌株对两种拟南芥植物的线虫生物周期停滞作用;野生型 (Col-0) 和剑蛋白突变体fra2 ,后者对南方根结线虫敏感。有趣的是,S. monomycini ATHUBA 220 和S. colombiensis ATHUBA 438 能够分别对 Col-0 和fra2中的南方根结线虫生物周期产生负面影响,并在感染南方根结线虫后增加 Col-0 中的生长。然而,它们对爪哇分枝杆菌无效。Fra2植物也被证明对爪哇小孢子虫感染敏感,在用链霉菌菌株处理后生长减少。讨论了所选链霉菌菌株的杀线虫作用和植物生长调节能力。
更新日期:2020-05-30
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