Selective and sensitive molecular probes for hydrogen peroxide (H₂O₂), which plays diverse roles in oxidative stress and redox signaling, are urgently needed to investigate the physiological and pathological effects of H₂O₂. A lack of reliable tools for in vivo imaging has hampered the development of H₂O₂ mediated therapeutics. By combining a specific tandem Payne/Dakin reaction with a chemiluminescent scaffold, H₂O₂‐CL‐510 was developed as a highly selective and sensitive probe for detection of H₂O₂ both in vitro and in vivo. A rapid 430‐fold enhancement of chemiluminescence was triggered directly by H₂O₂ without any laser excitation. Arsenic trioxide induced oxidative damage in leukemia was successfully detected. In particular, cerebral ischemia‐reperfusion injury‐induced H₂O₂ fluxes were visualized in rat brains using H₂O₂‐CL‐510 , providing a new chemical tool for real‐time monitoring of H₂O₂ dynamics in living animals.

中文翻译：

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用于细胞和动物中过氧化氢实时监测的高度选择性和灵敏的化学发光探针。

为了研究H ₂ O ₂的生理和病理作用，迫切需要选择性和灵敏的过氧化氢（H ₂ O ₂）分子探针，它们在氧化应激和氧化还原信号传导中起着不同的作用。缺乏用于体内成像的可靠工具已经阻碍了H ₂ O ₂介导的治疗剂的发展。通过将特定的串联Payne / Dakin反应与化学发光支架相结合，H ₂ O ₂ -CL-510被开发为用于检测H ₂ O ₂的高选择性和灵敏探针无论是体内还是体外。H ₂ O ₂无需任何激光激发即可直接触发化学发光的430倍快速增强。成功检测到三氧化二砷诱导的白血病的氧化损伤。特别是，使用H ₂ O ₂ -CL-510在大鼠大脑中观察了脑缺血再灌注损伤诱导的H ₂ O ₂通量，为实时监测活体动物中H ₂ O _2的动力学提供了新的化学工具。