Subcritical water hydrolysis (SWH) is a promising pre-treatment process for the production of second-generation ethanol (2 G ethanol), furthermore subcritical water is a fast, non-toxic, and green process. To address this issue SWH was performed at follow conditions: 200 °C, 15 MPa, 20 e 5 mL min^–1; the hydrolysate was obtained in order to produce a fermentable liquor in three different steps, 1) pure SWH, 2) SWH concentrated and rotary evaporator, and 3) SWH combinated with enzymatic hydrolysis. S. cerevisiae SA-1 and the pentose metabolizing yeast, S. stipitis NRRLY-7124, were used to evaluate fermentation potential. The combination of the products of SWH and the integration of enzymatic hydrolysis provided the greatest fermentable sugar yield with 3.0 ± 0.53 g L^–1 xylose and 6.3 ± 0.5 g L^–1 glucose; subsequent fermentation with S. cerevisiae SA-1 and S. stipitis NRRLY-7124 resulted in a 2 G ethanol yield of 2.20 ± 0.01 g L^–1.

亚临界水水解（SWH）是用于生产第二代乙醇（2 G乙醇）的一种有前途的预处理工艺，此外，亚临界水是一种快速，无毒且绿色的工艺。为了解决这个问题，SWH在以下条件下进行：200°C，15 MPa，20 e 5 mL min ^–1；为了在三个不同的步骤中生产可发酵的液体，获得了水解产物：1）纯SWH，2）SWH浓缩旋转蒸发仪，3）SWH与酶促水解结合。酿酒酵母SA-1和戊糖代谢酵母，葡萄球菌NRRLY-7124用于评估发酵潜力。SWH产物的结合和酶促水解的结合提供了最大的可发酵糖产量，其中葡萄糖为3.0±0.53 g L ^–1木糖和6.3±0.5 g L ^–1葡萄糖；随后的发酵与酿酒酵母SA-1和S.树干毕赤酵母NRRLY-7124导致的2.20±0.01克L-一2 G乙醇收率^-1。