Background

Cyclophosphamide (CP) is an alkylating chemotherapeutic drug used in the treatment of many types of cancer. However, as with other chemotherapeutic drugs, the use of CP is limited by the damage to healthy tissues such as testes, bladder and liver as well as cancerous tissue. Boron (B) is a trace element with many biological properties such as antioxidant, anti-apoptotic and anti-lipid per oxidation.

Methods

This current study aims to determine protective effects of B on CP induced testicular toxicity. The rats were divided into 4 groups (control, CP, B and B plus CP groups). The testes of experimental animals were taken for histological, apoptotic markers and biochemical analysis.

Results

The damage to some seminifer tubules, loss of typical appearance, thinning of seminifer epithelium and relative enlargement of the tubule lumen were watched in testis of the group that administrated CP. Moreover, Bcl-2, TAC and GSH levels decreased while TOC, OSI, MDA, Bax and Caspase-3 levels increased. On the other hand, pretreatment limited to B in the B plus CP group, testicular tissue improved. In addition, Bcl-2, GSH, TAC levels increased, Bax, MDA, TOC, OSI and caspase-3 levels decreased.

Conclusion

B significantly reduced testicular lipid per-oxidation and strengthened antioxidant defenses. Our results showed that pre-treatment B can protect rat testis against CP-induced testicular damage owing to its anti-lipid per oxidation, anti-oxidant and anti-apoptotic properties.

中文翻译：

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环磷酰胺诱导大鼠氧化应激、脂质过氧化、细胞凋亡和组织病理学变化：硼的保护作用。

背景

环磷酰胺 (CP) 是一种烷基化化疗药物，用于治疗多种癌症。然而，与其他化疗药物一样，CP 的使用受到对睾丸、膀胱和肝脏等健康组织以及癌组织的损害的限制。硼 (B) 是一种微量元素，具有许多生物学特性，例如抗氧化、抗凋亡和抗氧化脂质。

方法

本研究旨在确定 B 对 CP 诱导的睾丸毒性的保护作用。将大鼠分为4组（对照组、CP组、B组和B加CP组）。取实验动物的睾丸进行组织学、凋亡标志物和生化分析。

结果

CP组的睾丸观察到部分曲细精管的损伤、典型外观的丧失、曲细精管上皮变薄和小管腔相对增大。此外，Bcl-2、TAC 和 GSH 水平降低，而 TOC、OSI、MDA、Bax 和 Caspase-3 水平增加。另一方面，B加CP组中仅限于B的预处理，睾丸组织有所改善。此外，Bcl-2、GSH、TAC 水平升高，Bax、MDA、TOC、OSI 和 caspase-3 水平降低。

结论

B 显着降低睾丸脂质过氧化并加强抗氧化防御。我们的结果表明，预处理 B 可以保护大鼠睾丸免受 CP 诱导的睾丸损伤，因为它具有抗脂质过氧化、抗氧化和抗细胞凋亡的特性。