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Exploration and enrichment of methane-oxidizing bacteria derived from a rice paddy field emitting highly concentrated methane.
Journal of Bioscience and Bioengineering ( IF 2.8 ) Pub Date : 2020-05-30 , DOI: 10.1016/j.jbiosc.2020.04.006
Shohei Yasuda 1 , Risako Toyoda 1 , Shelesh Agrawal 2 , Toshikazu Suenaga 3 , Shohei Riya 1 , Tomoyuki Hori 4 , Susanne Lackner 2 , Masaaki Hosomi 1 , Akihiko Terada 5
Affiliation  

Methane-oxidizing bacteria (MOB) possess the metabolic potential to assimilate the highly potent greenhouse gas, CH4, and can also synthesize valuable products. Depending on their distinct and fastidious metabolic pathways, MOB are mainly divided into Type I and Type II; the latter are known as producers of polyhydroxyalkanoate (PHA). Despite the metabolic potential of MOB to synthesize PHA, the ecophysiology of MOB, especially under high CH4 flux conditions, is yet to be understood. Therefore, in this study, a rice paddy soil receiving a high CH4 flux from underground was used as an inoculum to enrich MOB using fed-batch operation, then the enriched Type II MOB were characterized. The transitions in the microbial community composition and CH4 oxidation rates were monitored by 16S rRNA gene amplicon sequencing and degree of CH4 consumption. With increasing incubation time, the initially dominant Methylomonas sp., affiliated with Type I MOB, was gradually replaced with Methylocystis sp., Type II MOB, resulting in a maximum CH4 oxidation rate of 1.40 g-CH4/g-biomass/day. The quantification of functional genes encoding methane monooxygenase, pmoA and PHA synthase, phaC, by quantitative PCR revealed concomitant increases in accordance with the Type II MOB enrichment. These increases in the functional genes underscore the significance of Type II MOB to mitigate greenhouse gas emission and produce PHA.



中文翻译:

探索并富集排放高浓度甲烷的稻田甲烷氧化细菌。

甲烷氧化细菌(MOB)具有代谢潜力,可以吸收高效的温室气体CH 4,并且还可以合成有价值的产品。根据它们独特而又挑剔的代谢途径,MOB主要分为I型和II型。后者被称为聚羟基链烷酸酯(PHA)的生产商。尽管MOB具有合成PHA的代谢潜力,但MOB的生态生理学,尤其是在高CH 4通量条件下,尚待了解。因此,在这项研究中,使用从地下接收到高CH 4通量的稻田土壤作为接种物,通过分批补料操作富集MOB,然后对富集的II型MOB进行了表征。微生物群落组成和CH 4的转变通过16S rRNA基因扩增子测序和CH 4消耗程度监测氧化速率。随着孵育时间的延长,最初与I型MOB相关的优势甲基单孢菌属物种逐渐被M.ollocystis sp。II型MOB取代,导致最大CH 4氧化速率为1.40 g-CH 4 / g-生物量/天。编码甲烷单加氧酶,pmoA和PHA合酶,phaC的功能基因的定量通过定量PCR显示,伴随II型MOB富集而增加。功能基因的这些增加强调了II型MOB减轻温室气体排放并产生PHA的重要性。

更新日期:2020-05-30
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