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Isolation and identification of potassium-solubilizing bacteria from Mikania micrantha rhizospheric soil and their effect on M. micrantha plants
Global Ecology and Conservation ( IF 4 ) Pub Date : 2020-05-30 , DOI: 10.1016/j.gecco.2020.e01141
Feng Sun , Qiaojing Ou , Nan Wang , Zi xuan Guo , Yuyi Ou , Na Li , Changlian Peng

Potassium is directly involved in plant cell turgor-driven cell elongation, this is important for promoting the expansion of invasive plants. Soil potassium-solubilizing microorganisms in some invaded ecosystems may promote plant invasion by increasing potassium availability. The aims of this study were to isolate and identify potassium-solubilizing bacteria and to evaluate their contribution to the solubilization of potassium from potassium-feldspar, and to the potassium uptake of Mikania micrantha. Potassium-solubilizing bacteria were isolated using solid Aleksandrov medium in abandoned orchards that have been highly invaded by M. micrantha for more than 10 years. Our results revealed that 18 strains of efficient bacteria were identified using liquid Aleksandrov medium. Among the isolates, the Burkholderia genus had the highest solubilizing ability (1.75 mg L−1). The results of the pot culture experiment showed that the plant biomass and potassium content of M. micrantha with potassium-solubilizing bacteria were higher than in the control without these bacteria. Incubation with potassium-solubilizing bacteria, especially GZ18, significantly increased the plant phosphorus content. More potassium was solubilized in treatments with potassium-solubilizing bacteria than in the control without bacteria. These results strongly suggest that potassium-solubilizing bacteria in the rhizosphere of M. micrantha could improve potassium solubilization and uptake, and contributed to their success as an invasive species.



中文翻译:

薇甘菊根际土壤中增溶钾的细菌的分离,鉴定及其对M. micrantha植物的影响

钾直接参与植物细胞膨胀驱动的细胞伸长,这对于促进入侵植物的扩增很重要。在某些入侵的生态系统中,土壤中溶解钾的微生物可能会通过增加钾的利用率来促进植物入侵。这项研究的目的是分离和鉴定增钾细菌,并评估它们对钾长石中钾的增溶以及对薇甘菊(Mikania micrantha)钾吸收的贡献。使用Aleksandrov固体培养基在被M. micrantha高度侵袭的废弃果园中分离增钾细菌超过十年。我们的结果表明,使用液态Aleksandrov培养基鉴定出18株高效细菌。在分离株中,伯克霍尔德菌属具有最高的增溶能力(1.75 mg L -1)。盆栽试验的结果表明,有增钾细菌的杀虫剂M. micrantha的植物生物量和钾含量高于没有这些细菌的对照。与增钾细菌,特别是GZ18一起孵育,可显着增加植物的磷含量。用增钾细菌处理的钾比没有细菌的对照溶解的钾多。这些结果强烈表明,根际中的增钾细菌micrantha micrantha可以改善钾的溶解和吸收,并有助于其作为入侵物种的成功。

更新日期:2020-05-30
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