Kiwifruit (Actinidia spp., Family Actinidiaceae) is a recently domesticated crop with highly endangered genetic resources in field genebanks and in the centre of diversity. Improved genotypes of several species in the genus have been commercialised and several other species are used in breeding programmes. Cryopreservation is considered a safe and cost-effective option for ex-situ conservation of clonal crops. In the current research we tested if the currently available method of droplet vitrification (DV) cryopreservation could be simplified whilst improving plant regeneration. Plant regeneration in the nine accessions belonging to A. chinensis var. chinensis, and A. chinensis var. deliciosa, A. arguta, A. macrosperma and A. polygama after cryopreservation ranged from 59 to 88% with the improved method where cold acclimation and antioxidant treatment steps were eliminated while sucrose pretreatment of shoot tips has been reduced to two steps in two days. Use of smaller (0.5–1 mm compared to 2 mm) in vitro shoot tips from younger (two weeks after subculture), fast-growing shoots and the use of liquid sucrose for shoot tip pretreatment are considered to be the reasons for this improved response. We also introduced the use of sodium dichloroisocyanurate as an alternative to household bleach as a sterilant for kiwifruit tissue culture establishment. The improved DV protocol is being used to cryopreserve valuable kiwifruit genetic resources with greater efficiency.

猕猴桃（猕猴桃spp。，Family Actinidiaceae）是一种最近驯化的作物，在田间种质库和多样性中心具有高度濒危的遗传资源。该属中几个物种的改良基因型已经商品化，并且其他几个物种也已用于育种程序。冷冻保存被认为是对克隆作物进行非原生境保存的一种安全且具有成本效益的选择。在当前的研究中，我们测试了在改善植物再生的同时，是否可以简化液滴玻璃化（DV）冷冻保存的现有方法。中华九个品种的9个品种的植株再生。中华和A. chinensis var。deliciosa，A。arguta，A。macrosperma和A. 冷冻保存后的polygama范围从59％到88％，其中改进的方法取消了冷驯化和抗氧化剂处理步骤，而蔗糖的茎尖预处理在两天内减少为两个步骤。从较小（传代培养后两周）开始，使用较小的（0.5–1 mm相比2 mm的毫米，相比2 mm的）体外芽尖，生长快的芽以及使用液体蔗糖预处理芽尖是这种响应改善的原因。我们还介绍了使用二氯异氰尿酸钠替代家用漂白剂作为猕猴桃组织培养设施的消毒剂。改进的DV协议被用于以更高的效率冷冻保存有价值的奇异果遗传资源。年轻（传代培养后两周）的体外芽梢为5–1 mm（相比2 mm），快速生长的芽以及使用液体蔗糖进行芽梢预处理被认为是这种响应改善的原因。我们还介绍了使用二氯异氰尿酸钠替代家用漂白剂作为猕猴桃组织培养设施的消毒剂。改进的DV协议被用于以更高的效率冷冻保存有价值的奇异果遗传资源。年轻（传代培养后两周）的体外芽梢为5–1 mm（相比2 mm），快速生长的芽以及使用液体蔗糖进行芽梢预处理被认为是这种响应改善的原因。我们还介绍了使用二氯异氰尿酸钠替代家用漂白剂作为猕猴桃组织培养设施的消毒剂。改进的DV协议被用于以更高的效率冷冻保存有价值的奇异果遗传资源。我们还介绍了使用二氯异氰尿酸钠替代家用漂白剂作为猕猴桃组织培养设施的消毒剂。改进的DV协议被用于以更高的效率冷冻保存有价值的奇异果遗传资源。我们还介绍了使用二氯异氰尿酸钠替代家用漂白剂作为猕猴桃组织培养设施的消毒剂。改进的DV协议被用于以更高的效率冷冻保存有价值的奇异果遗传资源。