Glycerol plasticized soy protein isolate (SPI) films at different contents (1 to 5% w/w w.r.t SPI) of nalidixic acid (NA) were fabricated by solution casting method. SPI films and NA incorporated SPI films were structurally and mechanically characterized by Fourier transform infrared spectroscopy (FTIR) and mechanical properties, respectively. Thermomechanical and water uptake studies were also carried out for NA incorporated SPI films. The results from FTIR study indicated the presence of NA in SPI as evidenced by generation of two additional peaks at 1259 cm^− 1 and 800 cm^− 1 attributed to C–C stretching vibration and the deformation of the heterocyclic aromatic ring, respectively. At 4% NA, the tensile stress and tensile modulus of SPI films increased from 5.18 MPa to 8.57 MPa and 57.14 MPa to 93.90 MPa, respectively. Antibacterial activity of NA incorporated SPI films against E. coli and L. monocytogenes were studied. The antibacterial studies of NA incorporated SPI film showed the clear zone of inhibition against both the bacteria. Docking studies were performed for SPI in order to evaluate their binding affinity with NA and similar type of aromatic acids such as mandelic and benzilic acid with which SPI films have been prepared earlier by our group. After docking, the ligands in form of aromatic acids were evaluated according to their binding energy and the interaction pattern between SPI and aromatic acids. The docking results indicated that nitrogen containing heterocyclic ring moiety remained intact during interaction of SPI and NA. This work will be helpful in fabricating NA incorporated SPI film from the renewable resources with significant antibacterial properties and reasonable mechanical properties.

采用溶液流延法制备了不同含量的萘啶酸（NA）（1〜5％w / w wrt SPI）的甘油增塑大豆分离蛋白（SPI）膜。通过傅立叶变换红外光谱（FTIR）和机械性能分别对SPI膜和掺有NA的SPI膜进行结构和机械表征。还对掺入NA的SPI膜进行了热机械和吸水研究。FTIR研究的结果表明，SPI中存在NA，这可以通过在1259 cm ^-1和800 cm ^-1处产生两个附加峰来证明分别归因于CC拉伸振动和杂环芳环的变形。在4％NA下，SPI薄膜的拉伸应力和拉伸模量分别从5.18 MPa增加到8.57 MPa和57.14 MPa增加到93.90 MPa。NA掺入的SPI薄膜对大肠杆菌和单核细胞增生李斯特菌的抗菌活性被研究了。掺入NA的SPI膜的抗菌研究表明，对两种细菌都有明显的抑制作用。为了评估SPI与NA和类似类型的芳香酸（如扁桃酸和苯甲酸）的结合亲和力，我们进行了对接研究，早先由我们小组制备了SPI膜。停靠后，根据芳族酸的结合能以及SPI与芳族酸之间的相互作用模式评估芳族酸形式的配体。对接结果表明，含氮杂环部分在SPI和NA相互作用期间保持完整。这项工作将有助于从具有显着的抗菌性能和合理的机械性能的可再生资源制备掺有NA的SPI膜。