Polyhydroxyalkanoate (PHA) is a biodegradable polyoxoester, which is synthesized as carbon and energy storage by various bacteria from different substrates used. Palm oil mill effluent (POME) has been redefined as a secondary raw material for PHA production. POME is bioconverted to volatile fatty acids (VFA) via anaerobic acidogenic fermentation, and VFA is utilized to synthesis PHA aerobically. A pH of 7.0 is the optimal condition to produce the highest VFA concentration with the lowest strength of formic acid. A mixed culture of aerobic activated sludge facilitates higher PHA production yield than a pure Bacillus megaterium culture. The feast–famine (FF) feeding strategy can also generate higher PHA yield compared with batch operation. Hence, the highest PHA yield of 0.2559 ± 0.0896 g PHA/g TVFA from POME is achieved by the cultivation of aerobic activated sludge under FF regime. The PHA production can be lowered by presence of formic acid and sludge biomass residue in the fermented POME.

聚羟基链烷酸酯（PHA）是一种可生物降解的聚氧酸酯，它是由各种细菌从不同的底物上合成为碳和能量的。棕榈油厂废水（POME）已被重新定义为生产PHA的次要原料。通过厌氧产酸发酵将POME生物转化为挥发性脂肪酸（VFA），并利用VFA需氧合成PHA。pH 7.0是产生最高VFA浓度和最低甲酸强度的最佳条件。有氧活性污泥的混合培养比纯巨大芽孢杆菌可促进更高的PHA产量文化。与分批操作相比，节食（FF）喂养策略还可产生更高的PHA产量。因此，通过在FF模式下培养好氧活性污泥可实现POME的最高PHA产量0.2559±0.0896 g PHA / g TVFA。发酵的POME中存在甲酸和污泥生物质残留物可以降低PHA的产量。