Abstract

Tricaine mesylate, also known as MS-222, was investigated to characterize its effects on sensory neurons, synaptic transmission at the neuromuscular junction, and heart rate in invertebrates. Three species were examined: Drosophila melanogaster, blue crab (Callinectes sapidus), and red swamp crayfish (Procambarus clarkii). Intracellular measures of action potentials in motor neurons of the crayfish demonstrated that MS-222 dampened the amplitude, suggesting that voltage-gated Na + channels are blocked by MS-222. This is likely the mechanism behind the reduced activity measured in sensory neurons and depressed synaptic transmission in all three species as well as reduced cardiac function in the larval Drosophila. To address public access to data, a group effort was used for analysis of given data sets, blind to the experimental design, to gauge analytical accuracy. The determination of a threshold in analysis for measuring extracellular recorded sensory events is critical and is not easily performed with commercial software.

Graphic abstract

中文翻译：

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甲磺酸三卡因对节肢动物的影响：小龙虾，蟹和果蝇。

摘要

甲磺酸三卡因（也称为MS-222）已被研究以表征其对感觉神经元，神经肌肉接头处的突触传递和无脊椎动物心率的影响。检查了三个物种：果蝇，蓝蟹（Callinectes sapidus）和红色沼泽小龙虾（Procambarus clarkii）。小龙虾运动神经元中动作电位的细胞内测量表明，MS-222减弱了振幅，表明电压门控的Na +通道被MS-222阻断。这可能是所有三种物种的感觉神经元活动降低和突触传递降低以及幼虫果蝇心脏功能降低背后的机制。。为了解决公众对数据的访问问题，人们花了很多心血来分析给定的数据集，而对实验设计却视而不见，以评估分析的准确性。在用于测量细胞外记录的感觉事件的分析中确定阈值是至关重要的，并且使用商业软件不容易执行。

图形摘要