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Improving the accuracy of coliform detection in meat products using modified dry rehydratable film method.
Food Science and Biotechnology ( IF 2.9 ) Pub Date : 2020-05-30 , DOI: 10.1007/s10068-020-00778-8
Ju-Young Kang 1 , Seo-Hyun Lee 1 , Ah-Hyeon Jo 1 , Eun-Ji Park 1 , Young-Seok Bak 2 , Jung-Beom Kim 1
Affiliation  

This study was undertaken to improve the detection accuracy for coliform bacteria, by analyzing biochemical properties of false positive and false negative colonies isolated from two dry rehydratable film methods, 3 M™ Petrifilm™ E. coli/Coliform count (PCC) and MC-Media Pad coliform count (MCC). The detection accuracy of PCC and MCC was determined to be 99.4% and 97.9%, respectively, with the detection error being 0.6% and 2.1%, respectively. False positive colonies (red colony without gas) on PCC were identified as Hafnia alvei and Enterobacter cloacae. All false positive colonies on MCC were identified as Aeromonas caviae; this organism gives a positive oxidase test, whereas coliform bacteria are oxidase negative. In conclusion, we propose that for improving detection accuracy of coliform bacteria, the incubation time of PCC should be modified and increased from 24 h to 48 h, and the oxidase test of MCC isolates should be included in the Korea Food Code.



中文翻译:

使用改良的干式可再水化膜方法提高肉类产品中大肠菌群检测的准确性。

通过分析从两种干燥的可水化膜方法,3 M™Petrifilm™大肠杆菌/大肠菌群计数(PCC)和MC-Media分离出的假阳性和假阴性菌落的生化特性,来提高大肠菌的检测准确性。垫大肠菌群计数(MCC)。PCC和MCC的检测准确度分别确定为99.4%和97.9%,检测误差分别为0.6%和2.1%。PCC上的假阳性菌落(无气体的红色菌落)被鉴定为Hafnia alvei阴沟肠杆菌。MCC上所有假阳性菌落均被鉴定为豚鼠气单胞菌; 这种生物的氧化酶测试结果呈阳性,而大肠菌则为氧化酶阴性。总之,我们建议为了提高大肠菌群的检测准确性,应修改PCC的孵育时间并将其从24 h增加到48 h,并将MCC分离物的氧化酶测试纳入韩国食品法规。

更新日期:2020-05-30
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